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Invitrogen
Phospho-Calmodulin 1/2/3 (Thr80, Ser82) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-Calmodulin 1/2/3 (Thr80, Ser82) Antibody (PA5-104932) in ICC/IF
Immunofluorescent analysis of Phospho-Calmodulin 3 (Thr79, Ser81) in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-Calmodulin 3 (Thr79, Ser81) polyclonal antibody (Product # PA5-104932) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-104932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CALM1(Accession P0DP23), corresponding to amino acid residues around phosphorylated Ser82.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Calmodulin1/2/3 only when phosphorylated at Thr80 + Ser82, which site historically referenced as Thr79+Ser81.
Target Information
Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CALM2; calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); Calmodulin III; Calmodulin-1; Calmodulin-2; Calmodulin-3; caM; epididymis secretory protein Li 72; LP7057 protein; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase subunit delta 1; phosphorylase kinase subunit delta 2; phosphorylase kinase subunit delta 3; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3; unnamed protein product
Gene Aliases: 1500001E21Rik; AI256814; AI327027; AI461935; AL024000; AL024017; CALM; CALM1; CALM2; CALM3; CALML2; CAM; CAM1; CAM2; CAM3; CaMA; CAMB; CAMC; CAMI; CAMII; CAMIII; CPVT4; CPVT6; DD132; HEL-S-72; LQT14; LQT15; LQT16; PHKD; PHKD1; PHKD2; PHKD3; R75142; RNCAMIII
UniProt ID: (Human) P0DP23, (Rat) P0DP29, (Mouse) P0DP26, (Mouse) P0DP27, (Rat) P0DP31
Entrez Gene ID: (Human) 801, (Human) 805, (Human) 808, (Rat) 24242, (Mouse) 12313, (Rat) 50663, (Mouse) 12314, (Rat) 24244, (Mouse) 12315
calcium channel regulator activity
calcium ion binding
protein binding
adenylate cyclase activator activity
calcium channel inhibitor activity
protein kinase binding
titin binding
protein serine/threonine kinase activator activity
ion channel binding
metal ion binding
calcium-dependent protein binding
protein phosphatase activator activity
transporter inhibitor activity
adenylate cyclase binding
protein domain specific binding
nitric-oxide synthase regulator activity
type 3 metabotropic glutamate receptor binding
phosphatidylinositol 3-kinase binding
nitric-oxide synthase binding
enzyme regulator activity
thioesterase binding
N-terminal myristoylation domain binding
phospholipase binding
protein N-terminus binding
calmodulin-related
G2/M transition of mitotic cell cycle
regulation of heart rate
detection of calcium ion
G-protein coupled receptor signaling pathway
JAK-STAT cascade
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
autophagosome membrane docking
substantia nigra development
regulation of cytokinesis
cellular response to interferon-beta
positive regulation of JAK-STAT cascade
regulation of calcium-mediated signaling
response to calcium ion
regulation of cardiac muscle contraction
long-term synaptic potentiation
regulation of ryanodine-sensitive calcium-release channel activity
negative regulation of ryanodine-sensitive calcium-release channel activity
cellular response to interferon-gamma
calcineurin-mediated signaling
regulation of cardiac muscle cell action potential
organelle localization by membrane tethering
presynaptic endocytosis
negative regulation of high voltage-gated calcium channel activity
regulation of cell communication by electrical coupling involved in cardiac conduction
negative regulation of calcium ion export across plasma membrane
mitochondrion-ER tethering
response to amphetamine
response to corticosterone
establishment of protein localization to membrane
establishment of protein localization to mitochondrial membrane
regulation of synaptic vesicle endocytosis
regulation of synaptic vesicle exocytosis
activation of adenylate cyclase activity
calcium-mediated signaling
positive regulation of cyclic nucleotide metabolic process
positive regulation of phosphoprotein phosphatase activity
positive regulation of protein dephosphorylation
positive regulation of DNA binding
positive regulation of nitric-oxide synthase activity
positive regulation of cyclic-nucleotide phosphodiesterase activity
positive regulation of ryanodine-sensitive calcium-release channel activity
regulation of store-operated calcium channel activity
regulation of high voltage-gated calcium channel activity
regulation of catalytic activity
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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