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          • Primary Antibodies ›
          • SMAD3 Antibodies

          Invitrogen

          Phospho-Smad3 (Thr179) Polyclonal Antibody

          View all (72) SMAD3 antibodies

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          Cite Phospho-Smad3 (Thr179) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          Phospho-Smad3 (Thr179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-Smad3 (Thr179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-Smad3 (Thr179) Antibody (PA5-104940) in IHC (P)

          Immunohistochemistry analysis of paraffin-embedded Phospho-Smad3 (Thr179) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Smad3 (Thr179) polyclonal antibody (Product # PA5-104940) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-Smad3 (Thr179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-Smad3 (Thr179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-Smad3 (Thr179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-Smad3 (Thr179) Antibody in Western Blot (WB)
          Phospho-Smad3 (Thr179) Polyclonal Antibody

          Product Details

          PA5-104940

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human SMAD3(Accession P84022), corresponding to amino acid residues around phosphorylated Thr179.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2816413

          Product Specific Information

          Antibody detects endogenous levels of Smad3 only when phosphorylated at Threonine 179.

          Target Information

          SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: DKFZP586N0721; DKFZp686J10186; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; unnamed protein product

          View more View less

          Gene Aliases: AU022421; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; Smad 3; SMAD3

          View more View less

          UniProt ID: (Human) P84022, (Mouse) Q8BUN5, (Rat) P84025

          View more View less

          Entrez Gene ID: (Human) 4088, (Mouse) 17127, (Rat) 25631

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding collagen binding beta-catenin binding zinc ion binding ligand-dependent nuclear receptor binding DEAD/H-box RNA helicase binding enzyme binding protein kinase binding phosphatase binding chromatin DNA binding ubiquitin protein ligase binding mineralocorticoid receptor binding sterol response element binding glucocorticoid receptor binding identical protein binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding sequence-specific DNA binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding co-SMAD binding I-SMAD binding R-SMAD binding DNA-binding transcription factor binding promoter-specific chromatin binding transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, protein binding RNA polymerase II activating transcription factor binding transcription factor binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity transcription regulatory region DNA binding SMAD binding protein heterodimerization activity DNA-binding transcription factor gene-specific transcriptional regulator
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter MAPK cascade skeletal system development osteoblast differentiation ureteric bud development response to hypoxia in utero embryonic development mesoderm formation somitogenesis release of cytochrome c from mitochondria liver development heart looping osteoblast development immune system development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process immune response transforming growth factor beta receptor signaling pathway JNK cascade gastrulation endoderm development cell proliferation negative regulation of cell proliferation anatomical structure morphogenesis embryonic pattern specification positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression cell differentiation negative regulation of ossification negative regulation of cell growth adrenal gland development positive regulation of cell migration positive regulation of bone mineralization thyroid gland development primary miRNA processing positive regulation of chondrocyte differentiation developmental process positive regulation of interleukin-1 beta production regulation of transforming growth factor beta2 production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway negative regulation of osteoblast proliferation nodal signaling pathway wound healing T cell activation negative regulation of protein catabolic process positive regulation of protein import into nucleus negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process cell-cell junction organization positive regulation of nitric oxide biosynthetic process negative regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response protein stabilization positive regulation of positive chemotaxis negative regulation of cytosolic calcium ion concentration positive regulation of stress fiber assembly regulation of mitochondrial membrane potential positive regulation of focal adhesion assembly pericardium development transdifferentiation positive regulation of SMAD protein import into nucleus SMAD protein signal transduction negative regulation of wound healing trophoblast cell migration negative regulation of lung blood pressure lens fiber cell differentiation cellular response to glucose stimulus cellular response to growth factor stimulus response to transforming growth factor beta cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway apoptotic signaling pathway extrinsic apoptotic signaling pathway cellular response to virus transforming growth factor beta receptor superfamily signaling pathway positive regulation of extracellular matrix assembly regulation of miRNA transcription negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac muscle hypertrophy in response to stress response to angiotensin negative regulation of protein phosphorylation transcription, DNA-templated transcription from RNA polymerase II promoter transport activation of cysteine-type endopeptidase activity involved in apoptotic process cell cycle arrest SMAD protein complex assembly positive regulation of alkaline phosphatase activity evasion or tolerance of host defenses by virus positive regulation of catenin import into nucleus intracellular signal transduction positive regulation of transcription factor import into nucleus negative regulation of mitotic cell cycle regulation of binding activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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