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Invitrogen
Phospho-BLNK (Tyr96) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-BLNK (Tyr96) Antibody (PA5-105018) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-BLNK (Tyr96) in human lymph node tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-BLNK (Tyr96) polyclonal antibody (Product # PA5-105018) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105018
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BLNK(Accession Q8WV28), corresponding to amino acid residues around phosphorylated Tyr96.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of BLNK only when phosphorylated at Tyrosine 96.
Target Information
B cell linker protein (Blnk) is a protein encoded by the Blnk gene located on chromosome 10 in humans. It is also known as SLP 65 (Src homology 2 domain-containing leukocyte protein of 65 kDa). It derives its name from being an adaptor protein in the B cell receptor signaling cascade with several supporting studies showing improper development of B cells in the absence of Blnk. Blnk undergoes tyrosine phosphorylation by Syk, a B cell receptor activated kinase, which provides a platform for interaction of signaling intermediaries such as the Vav GEF and Grb2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell adaptor containing SH2 domain; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; Cytoplasmic adapter protein; Lymphocyte antigen 57; MGC111051; SLP-65; Src homology 2 domain-containing leukocyte protein of 65 kDa; Src homology [SH2] domain-containing leukocyte protein of 65 kD; unnamed protein product
Gene Aliases: AGM4; BASH; Bca; BLNK; BLNK-S; Ly-57; LY57; Lyw-57; SLP-65; SLP65
UniProt ID: (Human) Q8WV28, (Mouse) Q9QUN3
Entrez Gene ID: (Human) 29760, (Mouse) 17060
transmembrane receptor protein tyrosine kinase adaptor activity
protein binding
lipid binding
enzyme binding
protein kinase binding
signaling adaptor activity
SH2 domain binding
phospholipase binding
molecular condensate scaffold activity
protein tyrosine kinase binding
SH3/SH2 adaptor activity
scaffold/adaptor protein
inflammatory response
humoral immune response
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of gene expression
B cell differentiation
intracellular signal transduction
B cell activation
B cell receptor signaling pathway
immune response
positive regulation of signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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