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Invitrogen

Phospho-NLRP3 (Ser295) Polyclonal Antibody

3 Published Figures
5 References
View all (32) NLRP3 antibodies

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Phospho-NLRP3 (Ser295) Polyclonal Antibody

Product Details

PA5-105071

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NLRP3(Accession Q96P20), corresponding to amino acid residues around phosphorylated Ser295.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816544

Product Specific Information

Antibody detects endogenous levels of NLRP3 only when phosphorylated at Ser295.

Target Information

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; cold auto-inflammatory syndrome 1; PYPAF1; NALP3; C1ORF7; cold autoinflammatory syndrome 1 homolog; cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1 protein homolog; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; FLJ95925; mast cell maturation inducible protein 1; Mast cell maturation-associated-inducible protein 1; member of the NALP family of inflammatory mediators; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NACHT/LRR/pyrin domain-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYD, NACHT, and LRR containing protein; PYRIN-containing APAF1-like protein 1; unnamed protein product

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Gene Aliases: AGTAVPRL; AII; AII/AVP; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; Mmig1; MWS; NALP3; NLRP3; PYPAF1

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UniProt ID: (Human) Q96P20, (Mouse) Q8R4B8

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Entrez Gene ID: (Human) 114548, (Mouse) 216799

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Function(s)
nucleotide binding protein binding ATP binding hydrolase activity ATPase activity protein binding, bridging signaling adaptor activity identical protein binding peptidoglycan binding ADP binding sequence-specific DNA binding binding, bridging phosphatidylinositol-4-phosphate binding DNA-binding transcription factor binding small molecule sensor activity cysteine-type endopeptidase activator activity molecular condensate scaffold activity phosphatidylinositol phosphate binding non-motor microtubule binding protein
Process(es)
pattern recognition receptor signaling pathway immune system process negative regulation of acute inflammatory response positive regulation of type 2 immune response regulation of transcription, DNA-templated apoptotic process defense response inflammatory response signal transduction osmosensory signaling pathway protein secretion detection of biotic stimulus response to virus negative regulation of interleukin-1 beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production positive regulation of interleukin-4 production interleukin-18-mediated signaling pathway NLRP3 inflammasome complex assembly innate immune response positive regulation of T-helper 2 cell differentiation positive regulation of transcription from RNA polymerase II promoter regulation of inflammatory response negative regulation of inflammatory response positive regulation of inflammatory response positive regulation of NF-kappaB transcription factor activity protein homooligomerization protein maturation pyroptosis interleukin-1-mediated signaling pathway cellular response to lipopolysaccharide cellular response to virus positive regulation of NLRP3 inflammasome complex assembly negative regulation of NIK/NF-kappaB signaling positive regulation of NIK/NF-kappaB signaling positive regulation of T-helper 2 cell cytokine production activation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of NF-kappaB transcription factor activity interleukin-1 beta production interleukin-18 production negative regulation of NF-kappaB import into nucleus positive regulation of cysteine-type endopeptidase activity involved in apoptotic process interleukin-1 secretion negative regulation of interleukin-1 beta secretion positive regulation of interleukin-1 beta secretion defense response to virus
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