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Invitrogen
Phospho-LIN28A (Ser200) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-LIN28A (Ser200) Antibody (PA5-105696) in ICC/IF
Immunofluorescent analysis of Phospho-LIN28A (Ser200) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-LIN28A (Ser200) polyclonal antibody (Product # PA5-105696) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor... View More
Product Details
PA5-105696
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LIN28A(Accession Q9H9Z2), corresponding to amino acid residues around phosphorylated Ser200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of LIN28A only when phosphorylated at Ser200.
Target Information
Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ12457; Lin-28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; unnamed protein product; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
Gene Aliases: AL024421; CSDD1; ENSMUSG00000070700; Gm10299; LIN-28; lin-28A; LIN28; LIN28A; Tex17; ZCCHC1
UniProt ID: (Human) Q9H9Z2, (Mouse) Q8K3Y3
Entrez Gene ID: (Human) 79727, (Mouse) 83557
germ cell development
miRNA metabolic process
miRNA catabolic process
posttranscriptional regulation of gene expression
negative regulation of translation
stem cell population maintenance
gene silencing by RNA
pre-miRNA processing
RNA 3'-end processing
positive regulation of TOR signaling
positive regulation of neuron differentiation
negative regulation of glial cell differentiation
positive regulation of translation
stem cell differentiation
positive regulation of protein kinase B signaling
regulation of gene silencing by miRNA
cellular response to glucose stimulus
positive regulation of cell proliferation involved in kidney development
negative regulation of pre-miRNA processing
positive regulation of cytoplasmic translation
regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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