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Invitrogen
Phospho-PFKFB2 (Ser466) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-PFKFB2 (Ser466) Antibody (PA5-106066) in IHC (P)
Immunohistochemistry analysis of Phospho-PFKFB2 (Ser466) in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-PFKFB2 (Ser466) polyclonal antibody (Product # PA5-106066) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PFKFB2(Accession O60825), corresponding to amino acid residues around phosphorylated Ser466.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PFKFB2 only when phosphorylated at Ser466.
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (heart); 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme; fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase; fructose-2,6-bisphosphatase, cardiac isozyme; PFK-2 car; PFK-2 cardiac; PFK-2/FBPase-2; PFK-2/FBPase-2 gene B; PFK/FBPase 2; PFKFB, cardiac; RH2K; unnamed protein product
Gene Aliases: 4930568D07Rik; PFK-2/FBPase-2; PFKFB2; RH2K
UniProt ID: (Human) O60825, (Mouse) P70265, (Rat) Q9JJH5
Entrez Gene ID: (Human) 5208, (Mouse) 18640, (Rat) 24640
fructose metabolic process
fructose 2,6-bisphosphate metabolic process
glucose catabolic process
lactate metabolic process
gluconeogenesis
glycolytic process
response to glucose
positive regulation of insulin secretion
carbohydrate phosphorylation
pyruvate metabolic process
dephosphorylation
positive regulation of glucokinase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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