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Invitrogen
GADD45A Polyclonal Antibody
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FIGURE: 1 / 2
GADD45A Antibody (PA5-106893) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded GADD45A in mouse brain tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with GADD45A polyclonal antibody (Product # PA5-106893) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-106893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GADD45A(Accession P24522), corresponding to amino acid residues M1-A43.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GADD45A.
Target Information
GADD45 alpha, beta and gamma are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents. GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase. GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DDIT 1; DDIT-1; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; DNA-damage inducible transcript 1; DNA-damage-inducible transcript 1; GADD45A2; Gadd45alpha; Growth arrest and DNA damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible, alpha; unnamed protein product
Gene Aliases: AA545191; DDIT1; GADD45; GADD45A
UniProt ID: (Human) P24522, (Mouse) P48316
Entrez Gene ID: (Human) 1647, (Mouse) 13197, (Rat) 25112
regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription from RNA polymerase II promoter
DNA repair
apoptotic process
cellular response to DNA damage stimulus
centrosome cycle
negative regulation of angiogenesis
negative regulation of peptidyl-serine phosphorylation of STAT protein
signal transduction in response to DNA damage
positive regulation of apoptotic process
negative regulation of blood vessel endothelial cell migration
positive regulation of JNK cascade
regulation of cell cycle
cellular response to mechanical stimulus
cellular response to ionizing radiation
negative regulation of protein serine/threonine kinase activity
positive regulation of p38MAPK cascade
positive regulation of reactive oxygen species metabolic process
activation of MAPKKK activity
negative regulation of protein kinase activity
response to stress
cell cycle
cell cycle arrest
mitotic cell cycle arrest
G2/M transition of mitotic cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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