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Invitrogen
EYA2 Polyclonal Antibody
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FIGURE: 1 / 1
EYA2 Antibody (PA5-109701) in WB
Western blot analysis of EYA2 in extracts of various cell lines. Samples were incubated with EYA2 Polyclonal antibody (Product # PA5-109701) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-109701
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human EYA2 (NP_005235.3).
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Amount
120 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: TSVRIGLMME EMIFNLADTH LFFNDLEDCD QIHVDDVSSD DNGQDLSTYN FSADGFHSSA PGANLCLGSG VHGGVDWMRK LAFRYRRVKE MYNTYKNNVG G
Target Information
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr- 142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Eyes absent homolog 2; probable transcription factor; Protein phosphatase EYA2; putative transcription factor; similar to Drosophila eyes absent gene; unnamed protein product
Gene Aliases: EAB1; EYA2
UniProt ID: (Human) O00167
Entrez Gene ID: (Human) 2139
DNA repair
chromatin organization
chromatin remodeling
cellular response to DNA damage stimulus
mesodermal cell fate specification
striated muscle tissue development
cell differentiation
positive regulation of DNA repair
extrinsic apoptotic signaling pathway in absence of ligand
mitochondrial outer membrane permeabilization
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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