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Invitrogen
RTF1 Polyclonal Antibody
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FIGURE: 1 / 1
RTF1 Antibody (PA5-110333) in WB
Western blot analysis of RTF1 in extracts of various cell lines. Samples were incubated with RTF1 Polyclonal antibody (Product # PA5-110333) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-110333
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human RTF1 (NP_055953.3).
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Amount
57 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LAKKQPLKTS EVYSDDEEEE EDDKSSEKSD RSSRTSSSDE EEEKEEIPPK SQPVSLPEEL NRVRLSRHKL ERWCHMPFFA KTVTGCFVRI GIGNHNSKPV Y
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gene trap locus 7; KIAA0252; ortholog of mouse gene trap locus 7; RNA polymerase-associated protein RTF1 homolog; Rtf1 Paf1/RNA polymerase II complex component homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog; unnamed protein product
Gene Aliases: 2900005O08Rik; 6530416A09Rik; AI853581; AU043144; AW553985; cbp82; GTL7; KIAA0252; RTF1
UniProt ID: (Human) Q92541, (Mouse) A2AQ19
Entrez Gene ID: (Human) 23168, (Mouse) 76246
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate commitment
blastocyst growth
chromatin organization
transcription elongation from RNA polymerase II promoter
Wnt signaling pathway
stem cell population maintenance
transcription, DNA-templated
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
histone modification
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of histone H3-K4 methylation
histone H3-K4 trimethylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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