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Invitrogen
BNIP3 Polyclonal Antibody
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FIGURE: 1 / 2
BNIP3 Antibody (PA5-11402) in ICC/IF
Immunocytochemistry analysis of BNIP3 in HepG2 cells. Samples were incubated with BNIP3 polyclonal antibody (Product # PA5-11402) using a dilution of 1:500 for 2 h at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:1,000 for 1hr. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
Product Details
PA5-11402
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 215-252 amino acids from human BNIP3
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Amount
480 µg
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19 kDa-interacting protein 1, NIP3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kDa-interacting protein 1, NIP3; BCL2/adenovirus E1B interacting protein 1, NIP3; BCL2/adenovirus E1B interacting protein 3; BNIP3; Hypoxia-Activated BNIP3 Overlapping Non-coding RNA; lacks exon 2; BNIP3deltaExon2; nineteen kD interacting protein-3; pro-apoptotic mitochondrial protein; unnamed protein product
Gene Aliases: BNIP3; HABON; NIP3
UniProt ID: (Human) Q12983, (Mouse) O55003
Entrez Gene ID: (Human) 664, (Mouse) 12176
response to hypoxia
apoptotic process
cell death
granzyme-mediated apoptotic signaling pathway
negative regulation of mitochondrial fusion
regulation of mitochondrion organization
mitochondrial protein catabolic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of protein complex disassembly
mitochondrial fragmentation involved in apoptotic process
negative regulation of membrane potential
regulation of mitochondrial membrane permeability
autophagic cell death
brown fat cell differentiation
neuron apoptotic process
defense response to virus
cellular response to hydrogen peroxide
cellular response to cobalt ion
cellular response to hypoxia
reactive oxygen species metabolic process
positive regulation of mitochondrial fission
positive regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
mitochondrial outer membrane permeabilization
toxin transport
regulation of aerobic respiration
intrinsic apoptotic signaling pathway in response to hypoxia
negative regulation of reactive oxygen species metabolic process
mitophagy
mitophagy
response to bacterium
positive regulation of autophagy
cardiac muscle cell apoptotic process
positive regulation of cardiac muscle cell apoptotic process
negative regulation of mitochondrial membrane potential
positive regulation of macroautophagy
cerebral cortex development
negative regulation of programmed cell death
response to axon injury
oligodendrocyte differentiation
positive regulation of mitochondrial calcium ion concentration
response to hyperoxia
reticulophagy
substrate localization to autophagosome
cellular response to mechanical stimulus
response to oxygen-glucose deprivation
granzyme-mediated programmed cell death signaling pathway
negative regulation of mitochondrial membrane permeability involved in apoptotic process
positive regulation of mitophagy
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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