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Invitrogen
Phospho-IKK alpha/beta (Ser176, Ser180) Polyclonal Antibody
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Product Details
PA5-114586
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IKK alpha/ beta around the phosphorylation site of Ser176/180.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IKK alpha/ beta only when phosphorylated at Ser176/180.
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKK alpha; IKK-1; IKK-2; IKK-A; IKK-a kinase; IKK-B; IKK-beta; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; nuclear factor kappa B kinase subunit beta; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; protein kinase; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16; unnamed protein product
Gene Aliases: AI132552; AI256658; AIM-1; BPS2; CHUK; Chuk1; Fbx24; Fbxo24; IKBKA; IKBKB; IKK-1; IKK-2; IKK-alpha; IKK-beta; IKK1; IKK2; IKK[b]; IKKA; IKKB; IKKbeta; IMD15; IMD15A; IMD15B; NFKBIKA; NFKBIKB; TCF16
UniProt ID: (Human) O15111, (Human) O14920, (Mouse) O88351, (Rat) Q9QY78
Entrez Gene ID: (Human) 1147, (Mouse) 12675, (Rat) 309361, (Human) 3551, (Mouse) 16150, (Rat) 84351
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
IkappaB kinase activity
kinase activity
transferase activity
protein homodimerization activity
macromolecular complex binding
protein heterodimerization activity
scaffold protein binding
transferrin receptor binding
receptor signaling protein serine/threonine kinase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
identical protein binding
protein serine kinase activity
non-receptor serine/threonine protein kinase
protein modifying enzyme
pattern recognition receptor signaling pathway
skeletal muscle contraction
regulation of transcription from RNA polymerase II promoter
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
response to xenobiotic stimulus
response to virus
response to toxic substance
anatomical structure morphogenesis
response to acetate
negative regulation of NF-kappaB transcription factor activity
positive regulation of interferon-alpha production
response to hydroperoxide
tumor necrosis factor-mediated signaling pathway
toll-like receptor 4 signaling pathway
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NF-kappaB transcription factor activity
striated muscle cell differentiation
response to cholecystokinin
cellular response to tumor necrosis factor
cellular response to virus
morphogenesis of an epithelial sheet
protein phosphorylation
I-kappaB phosphorylation
Rho protein signal transduction
lactation
response to organic substance
phosphorylation
signal transduction by protein phosphorylation
osteoclast differentiation
response to lipopolysaccharide
mammary gland epithelial cell proliferation
response to muscle stretch
odontogenesis of dentin-containing tooth
mammary gland alveolus development
response to drug
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
MyD88-dependent toll-like receptor signaling pathway
positive regulation of macromolecule metabolic process
regulation of tumor necrosis factor-mediated signaling pathway
peptidyl-serine phosphorylation
signal transduction involved in regulation of gene expression
regulation of toll-like receptor signaling pathway
toll-like receptor 3 signaling pathway
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription, DNA-templated
T cell receptor signaling pathway
stress-activated MAPK cascade
protein maturation
interleukin-1-mediated signaling pathway
protein localization to plasma membrane
negative regulation of cytokine production involved in inflammatory response
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
B cell homeostasis
positive regulation of cell proliferation
positive regulation of sodium ion transport
neuron projection development
serine phosphorylation of STAT protein
positive regulation of neuron death
positive regulation of cation channel activity
response to fatty acid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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