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Invitrogen
Phospho-Aconitase 1 (Ser711) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Aconitase 1 (Ser711) Antibody (PA5-114590) in ICC/IF
Immunocytochemistry analysis of Phospho-Aconitase 1 (Ser711) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114590). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488... View More
Product Details
PA5-114590
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IREB1 around the phosphorylation site of Ser711.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IREB1 only when phosphorylated at Ser711.
Target Information
Aconitase 1 (ACO1) also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aconitase; aconitase 1, soluble; aconitate hydratase, cytoplasmic; Citrate hydro-lyase; cytoplasmic aconitase; Cytoplasmic aconitate hydratase; cytosplasmic aconitase; epididymis luminal protein 60; epididymis secretory sperm binding protein; Ferritin repressor protein; IRE-BP 1; Iron regulatory protein 1; Iron-responsive element-binding protein 1; IRP1; OTTHUMP00000021176; OTTHUMP00000021177; OTTHUMP00000045233; RNA-binding protein; soluble aconitase
Gene Aliases: Aco-1; ACO1; Acon1; ACONS; AH; HEL60; IREB1; IREBP; IREBP1; IRP1
UniProt ID: (Human) P21399, (Rat) Q63270, (Mouse) P28271
Entrez Gene ID: (Human) 48, (Rat) 50655, (Mouse) 11428
translation repressor activity, nucleic acid binding
RNA binding
aconitate hydratase activity
protein binding
lyase activity
iron-responsive element binding
metal ion binding
iron-sulfur cluster binding
3 iron, 4 sulfur cluster binding
4 iron, 4 sulfur cluster binding
mRNA binding
mRNA 5'-UTR binding
RNA metabolism protein
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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