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Invitrogen
Apelin Polyclonal Antibody
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FIGURE: 1 / 9
Apelin Antibody (PA5-114860) in ICC/IF
Immunocytochemistry analysis of Apelin in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114860) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114860
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human APLN(Accession Q9ULZ1), corresponding to amino acid residues E35-F77.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Apelin.
Target Information
This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AGTRL1 ligand; Apelin; apelin, AGTRL1 ligand; APJ endogenous ligand; preproapelin; unnamed protein product
Gene Aliases: 6030430G11Rik; APEL; APLN; XNPEP2
UniProt ID: (Human) Q9ULZ1, (Mouse) Q9R0R4, (Rat) Q9R0R3
Entrez Gene ID: (Human) 8862, (Mouse) 30878, (Rat) 58812
regulation of the force of heart contraction
signal transduction
G-protein coupled receptor signaling pathway
feeding behavior
positive regulation of cell proliferation
positive regulation of heat generation
positive regulation of phosphorylation
drinking behavior
regulation of respiratory gaseous exchange
negative regulation of blood pressure
negative regulation of vasoconstriction
positive regulation of vasodilation
regulation of body fluid levels
positive regulation of corticotropin secretion
positive regulation of corticotropin-releasing hormone secretion
angiogenesis
G-protein coupled receptor internalization
negative regulation of systemic arterial blood pressure
immune response
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
gastrulation
lactation
positive regulation of heart rate
positive regulation of cardiac muscle hypertrophy
negative regulation of gene expression
negative regulation of fibroblast growth factor receptor signaling pathway
positive regulation of heart contraction
apelin receptor signaling pathway
coronary vasculature development
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of cardiac muscle hypertrophy in response to stress
positive regulation of cardiac muscle hypertrophy in response to stress
positive regulation of G protein-coupled receptor internalization
negative regulation of vascular smooth muscle cell proliferation
positive regulation of vascular endothelial cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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