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Invitrogen
CGRP Polyclonal Antibody
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FIGURE: 1 / 5
CGRP Antibody (PA5-114929) in ICC/IF
Immunocytochemistry analysis of CGRP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114929) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114929
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CALCA(Accession P06881), corresponding to amino acid residues G96-S116.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CALCA.
Target Information
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha-type CGRP; Calcitonin; calcitonin 1; calcitonin gene related protein; Calcitonin gene-related peptide 1; Calcitonin gene-related peptide I; calcitonin precursor; calcitonin/calcitonin-related polypeptide, alpha; CGRP-I; CGRP1; katacalcin; MGC126648; RAT calcitonin; unnamed protein product
Gene Aliases: CA; Cal1; CAL6; Calc; CALC1; CALCA; calcitonin; CGRP; CGRP-1; CGRP-alpha; CGRP-I; CGRP1; CT; Ctn; KC; PCT; RATCAL6
UniProt ID: (Human) P06881, (Rat) P01256, (Mouse) Q99JA0
Entrez Gene ID: (Human) 796, (Rat) 24241, (Mouse) 12310
response to yeast
endothelial cell proliferation
vasculature development
neurological system process involved in regulation of systemic arterial blood pressure
G-protein coupled receptor internalization
monocyte chemotaxis
protein phosphorylation
leukocyte cell-cell adhesion
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
embryo implantation
regulation of blood pressure
negative regulation of calcium ion transport into cytosol
antibacterial humoral response
antifungal humoral response
receptor internalization
activation of protein kinase activity
positive regulation of interleukin-1 alpha production
positive regulation of interleukin-8 production
vasodilation
endothelial cell migration
innate immune response
positive regulation of macrophage differentiation
negative regulation of osteoclast differentiation
negative regulation of blood pressure
negative regulation of bone resorption
negative regulation of transcription, DNA-templated
negative regulation of smooth muscle contraction
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
regulation of cytosolic calcium ion concentration
antimicrobial humoral immune response mediated by antimicrobial peptide
cellular response to tumor necrosis factor
calcitonin family receptor signaling pathway
amylin receptor 1 signaling pathway
amylin receptor 2 signaling pathway
cellular response to nerve growth factor stimulus
calcitonin gene-related peptide receptor signaling pathway
vasodilation of artery involved in baroreceptor response to increased systemic arterial blood pressure
regulation of heart rate
cellular calcium ion homeostasis
inflammatory response
neuropeptide signaling pathway
neuromuscular junction development
aging
feeding behavior
regulation of heart contraction
response to heat
negative regulation of ossification
positive regulation of cAMP biosynthetic process
negative regulation of neurological system process
positive regulation of adenylate cyclase activity
positive regulation of ossification
positive regulation of cell adhesion
positive regulation of vasodilation
response to pain
detection of temperature stimulus involved in sensory perception of pain
positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
sperm capacitation
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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