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Invitrogen
Nucleolin Polyclonal Antibody
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FIGURE: 1 / 5
Nucleolin Antibody (PA5-115675) in ICC/IF
Immunocytochemistry analysis of Nucleolin in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115675) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat a... View More
Product Details
PA5-115675
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NCL(Accession P19338), corresponding to amino acid residues D257-F307.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NUCL.
Target Information
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ45706; FLJ59041; nucl antibody; Nucleolin; nucleolin-like protein; Protein C23; unnamed protein product
Gene Aliases: B530004O11Rik; C23; D0Nds28; D1Nds28; NCL; Nsr1; Nuc; Nucl
UniProt ID: (Human) P19338, (Mouse) P09405
Entrez Gene ID: (Human) 4691, (Mouse) 17975, (Rat) 25135
nucleic acid binding
DNA binding
RNA binding
protein binding
telomeric DNA binding
PH domain binding
identical protein binding
insulin receptor substrate binding
DNA topoisomerase binding
mRNA 5'-UTR binding
nucleotide binding
single-stranded DNA binding
receptor binding
calcium ion binding
protein C-terminus binding
selenocysteine insertion sequence binding
rRNA primary transcript binding
histone binding
laminin binding
sequence-specific DNA binding
poly(A) RNA binding
ErbB-4 class receptor binding
angiogenesis
regulation of gene expression
positive regulation of macromolecule biosynthetic process
negative regulation of translation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin receptor signaling pathway
positive regulation of mRNA splicing, via spliceosome
regulation of RNA metabolic process
cellular response to epidermal growth factor stimulus
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
cellular response to leukemia inhibitory factor
endocytosis
positive regulation of tumor necrosis factor production
negative regulation of apoptotic process
cellular response to lipopolysaccharide
regulation of rRNA processing
positive regulation of interleukin-6 secretion
spermatogenesis
liver regeneration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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