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Invitrogen
PAF1 Polyclonal Antibody
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FIGURE: 1 / 2
PAF1 Antibody (PA5-115713) in ICC/IF
Immunocytochemistry analysis of PAF1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115713). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PAF1(Accession Q8N7H5), corresponding to amino acid residues M1-L39.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PAF1.
Target Information
This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPAF1; Paf1, RNA polymerase II associated factor, homolog; Pancreatic differentiation protein 2; RNA polymerase II-associated factor 1 homolog; unnamed protein product
Gene Aliases: 5730511K23Rik; AA675045; F23149_1; PAF1; PD2; RGD1306219
UniProt ID: (Human) Q8N7H5, (Rat) Q4V886, (Mouse) Q8K2T8
Entrez Gene ID: (Human) 54623, (Rat) 361531, (Mouse) 54624
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate commitment
transcription, DNA-templated
mRNA polyadenylation
biological_process
histone monoubiquitination
Wnt signaling pathway
stem cell population maintenance
positive regulation of mRNA 3'-end processing
positive regulation of transcription elongation from RNA polymerase II promoter
histone H2B ubiquitination
negative regulation of myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
regulation of transcription, DNA-templated
nucleosome positioning
positive regulation of histone methylation
protein localization to nucleus
transcription elongation from RNA polymerase II promoter
mRNA 3'-end processing
positive regulation of cell cycle G1/S phase transition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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