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Invitrogen
PAR1 Polyclonal Antibody
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FIGURE: 1 / 8
PAR1 Antibody (PA5-116040) in ICC/IF
Immunocytochemistry analysis of PAR1 in HepG2. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116040) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116040
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human F2R(Accession P25116), corresponding to amino acid residues M1-N47.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Thrombin Receptor.
Target Information
Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface G-protein coupled receptors which include PAR-1, PAR-2 and PAR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C HTR; Coagulation factor II receptor; PAR-1; PIG18A; protease-activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; unnamed protein product
Gene Aliases: AI482343; CF2R; F2R; HTR; PAR-1; PAR1; ThrR; TR; TRGPC
UniProt ID: (Human) P25116, (Mouse) P30558
Entrez Gene ID: (Human) 2149, (Mouse) 14062, (Rat) 25439
connective tissue replacement involved in inflammatory response wound healing
negative regulation of glomerular filtration
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
establishment of synaptic specificity at neuromuscular junction
blood coagulation
hemostasis
positive regulation of cell proliferation
negative regulation of cell proliferation
response to wounding
anatomical structure morphogenesis
glutamate secretion
platelet activation
regulation of blood coagulation
positive regulation of blood coagulation
positive regulation of cell migration
response to lipopolysaccharide
regulation of interleukin-1 beta production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of collagen biosynthetic process
positive regulation of Rho protein signal transduction
dendritic cell homeostasis
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
cell-cell junction maintenance
positive regulation of transcription, DNA-templated
positive regulation of vasoconstriction
positive regulation of smooth muscle contraction
positive regulation of JAK-STAT cascade
regulation of synaptic plasticity
homeostasis of number of cells within a tissue
release of sequestered calcium ion into cytosol
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
platelet dense granule organization
regulation of biological quality
positive regulation of ERK1 and ERK2 cascade
thrombin receptor signaling pathway
trans-synaptic signaling by endocannabinoid, modulating synaptic transmission
negative regulation of renin secretion into blood stream
activation of MAPKK activity
activation of cysteine-type endopeptidase activity involved in apoptotic process
protein kinase C-activating G-protein coupled receptor signaling pathway
tyrosine phosphorylation of STAT protein
STAT protein import into nucleus
positive regulation of phosphatidylinositol 3-kinase signaling
obsolete death
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
regulation of sensory perception of pain
positive regulation of interleukin-8 secretion
positive regulation of interleukin-6 secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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