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Invitrogen
ZDHHC20 Polyclonal Antibody
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FIGURE: 1 / 1
ZDHHC20 Antibody (PA5-116796) in WB
Western blot analysis of ZDHHC20 in extracts of various cell lines. Samples were incubated with ZDHHC20 Polyclonal antibody (Product # PA5-116796) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-116796
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 270-365 of human ZDHHC20 (NP_0013169881)
Conjugate
Form
Liquid
Concentration
2.54 mg/mL
Amount
254 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, MCF7, SKOV3, A-549, Mouse brain, Mouse lung, Mouse testis, Rat brain, Rat lung
Immunogen sequence: VFGDEKKYWL LPIFSSLGDG CSFPTRLVGM DPEQASVTNQ NEYARSSGSN QPFPIKPLSE SKNRLLDSES QWLENGAEEG IVKSGTNNHV TVAIEN
Target Information
Catalyzes palmitoylation of Cys residues on target proteins (PubMed:27153536, PubMed:29326245). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acyltransferase ZDHHC20; DHHC domain-containing cysteine-rich protein 20; DHHC-20; DHHC-containing protein 20; DHHC20; membrane-associated DHHC24 zinc finger protein; Palmitoyltransferase ZDHHC20; probable palmitoyltransferase ZDHHC20; unnamed protein product; Zinc finger DHHC domain-containing protein 20; zinc finger DHHC-type containing 20; zinc finger DHHC-type palmitoyltransferase 20; zinc finger, DHHC domain containing 20
Gene Aliases: 4933421L13Rik; 5033406L14Rik; AI448102; B230110O18Rik; DHHC-20; DHHC20; HK11; RGD1305755; ZDHHC20
UniProt ID: (Human) Q5W0Z9, (Mouse) Q5Y5T1
Entrez Gene ID: (Human) 253832, (Rat) 305923, (Mouse) 75965
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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