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Invitrogen

Phospho-SHP-1 (Tyr564) Polyclonal Antibody

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Phospho-SHP-1 (Tyr564) Polyclonal Antibody

Product Details

PA5-118523

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PTPN6(Accession P29350), corresponding to amino acid residues around phosphorylated Tyr564.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2903025

Product Specific Information

Antibody detects endogenous levels of SHP1 only when phosphorylated at Tyr564.

Target Information

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 70Z-SHP; EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; motheaten; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; PTPTY-42; SH-PTP1; SH2 phosphatase 1; SHP-1; Tyrosine-protein phosphatase non-receptor type 6; unnamed protein product

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Gene Aliases: 70Z-SHP; HCP; HCPH; HPTP1C; me; PTP-1C; PTP1C; Ptph6; PTPN6; PTPTY-42; SH-PTP1; SHP-1; SHP-1L; SHP1

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UniProt ID: (Human) P29350, (Rat) P81718, (Mouse) P29351

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Entrez Gene ID: (Human) 5777, (Rat) 116689, (Mouse) 15170

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Function(s)
phosphotyrosine binding phosphoprotein phosphatase activity protein tyrosine phosphatase activity non-membrane spanning protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding hydrolase activity SH3 domain binding protein kinase binding SH2 domain binding cell adhesion molecule binding phosphorylation-dependent protein binding natural killer cell lectin-like receptor binding phosphatase activity protein phosphatase
Process(es)
MAPK cascade mitotic cell cycle pattern recognition receptor signaling pathway hematopoietic progenitor cell differentiation neutrophil activation involved in immune response cytoplasmic pattern recognition receptor signaling pathway negative regulation of humoral immune response mediated by circulating immunoglobulin protein dephosphorylation G-protein coupled receptor signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of angiogenesis peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway cell differentiation platelet formation T cell costimulation negative regulation of lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production negative regulation of mast cell activation involved in immune response positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine dephosphorylation intracellular signal transduction megakaryocyte development T cell proliferation T cell activation negative regulation of T cell proliferation natural killer cell mediated cytotoxicity regulation of apoptotic process negative regulation of MAPK cascade regulation of B cell differentiation positive regulation of angiogenesis negative regulation of innate immune response T cell receptor signaling pathway B cell receptor signaling pathway negative regulation of B cell receptor signaling pathway negative regulation of T cell receptor signaling pathway regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling regulation of type I interferon-mediated signaling pathway regulation of ERK1 and ERK2 cascade platelet aggregation inflammatory response to wounding negative regulation of inflammatory response to wounding CD27 signaling pathway positive regulation of cytokine production involved in inflammatory response negative regulation of neutrophil activation epididymis development regulation of G1/S transition of mitotic cell cycle cell proliferation response to wounding positive regulation of phosphatidylinositol 3-kinase signaling abortive mitotic cell cycle negative regulation of MAP kinase activity negative regulation of peptidyl-tyrosine phosphorylation signal transduction dephosphorylation
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