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Invitrogen
CKMT2 Polyclonal Antibody
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FIGURE: 1 / 4
CKMT2 Antibody (PA5-118822) in ICC/IF
Immunocytochemical analysis of CKMT2 in Hela cells using a CKMT2 polyclonal antibody (Product # PA5-118822). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with the primary antibody and mouse anti-beta tubulin antibody at a dilution of 1:200 for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear cou... View More
Product Details
PA5-118822
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CKMT2(Accession P17540), corresponding to amino acid residues A242-K292.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CKMT2.
Target Information
Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic-type mitochondrial creatine kinase; CKMT2; Creatine kinase S-type, mitochondrial; creatine kinase, mitochondrial 2 (sarcomeric); EC 2.7.3.2; Mib-CK; S-MtCK; Sarcomeric mitochondrial creatine kinase; sarcomeric mitochondrial creatine kinase precursor (EC 2.7.3.2); unnamed protein product
Gene Aliases: 2300008A19Rik; CKMT2; ScCKmit; SMTCK
UniProt ID: (Human) P17540, (Mouse) Q6P8J7
Entrez Gene ID: (Human) 1160, (Rat) 688698, (Mouse) 76722
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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