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Invitrogen
Nodal Polyclonal Antibody
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FIGURE: 1 / 3
Nodal Antibody (PA5-119761) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human colon tissue using Nodal Polyclonal Antibody (Product # PA5-119761). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Nodal antibody at a dilution of 1... View More
Product Details
PA5-119761
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human Nodal aa 150-347.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Rat testis tissue cell lysate, SHSY5Y cell lysate, rat testis tissue, human colon tissue.
Subcellular Location: Secreted.
Target Information
NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC138230; Nodal homolog
Gene Aliases: HTX5; NODAL
UniProt ID: (Human) Q96S42
Entrez Gene ID: (Human) 4838, (Rat) 294503
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
gastrulation with mouth forming second
formation of primary germ layer
mesoderm formation
trophectodermal cell differentiation
trophectodermal cellular morphogenesis
neural fold formation
liver development
placenta development
embryonic placenta development
maternal placenta development
vasculature development
heart looping
inhibition of neuroepithelial cell differentiation
determination of left/right asymmetry in lateral mesoderm
transmembrane receptor protein serine/threonine kinase signaling pathway
transforming growth factor beta receptor signaling pathway
germ cell development
determination of left/right symmetry
gastrulation
nervous system development
brain development
endoderm development
heart development
female pregnancy
cell proliferation
positive regulation of cell proliferation
embryonic pattern specification
anterior/posterior axis specification
anterior/posterior pattern specification
regulation of signal transduction
polarity specification of proximal/distal axis
regulation of gastrulation
positive regulation of vascular endothelial growth factor production
negative regulation of cell development
cell migration
stem cell population maintenance
positive regulation of cell-cell adhesion
cell differentiation
lung development
floor plate morphogenesis
somatic stem cell population maintenance
embryonic heart tube development
endodermal cell differentiation
nodal signaling pathway
cell migration involved in gastrulation
cell fate commitment
negative regulation of cell differentiation
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
axial mesodermal cell fate specification
mesendoderm development
digestive tract morphogenesis
anatomical structure formation involved in morphogenesis
embryonic cranial skeleton morphogenesis
tissue morphogenesis
formation of anatomical boundary
positive regulation of epithelial cell proliferation
digestive system development
embryonic process involved in female pregnancy
maternal process involved in parturition
positive regulation of SMAD protein import into nucleus
left lung morphogenesis
negative regulation of androgen receptor signaling pathway
epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification
positive regulation of ERK1 and ERK2 cascade
primitive streak formation
apoptotic signaling pathway
negative regulation of trophoblast cell migration
negative regulation of chorionic trophoblast cell proliferation
regulation of stem cell population maintenance
negative regulation of reproductive process
positive regulation of pathway-restricted SMAD protein phosphorylation
positive regulation of activin receptor signaling pathway
growth
regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of MAPK cascade
cell development
positive regulation of sequence-specific DNA binding transcription factor activity
SMAD protein signal transduction
transforming growth factor beta receptor signaling pathway involved in primitive streak formation
nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
positive regulation of receptor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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