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          • Primary Antibodies ›
          • Presenilin 1 Antibodies

          Invitrogen

          Presenilin 1 Polyclonal Antibody

          View all (33) Presenilin 1 antibodies

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          Cite Presenilin 1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Presenilin 1 Antibody (PA5-119872) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of of Presenilin 1/PS-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with Presenilin 1 Polyclonal Antibody (Product # PA5-119872) at a dilution of 1:50 for 1 hour at room temperature,... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)
          Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Presenilin 1 Antibody in Western Blot (WB)
          Presenilin 1 Antibody in Western Blot (WB)
          Presenilin 1 Polyclonal Antibody

          Product Details

          PA5-119872

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          -

          Flow Cytometry (Flow)

          1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide within human Presenilin 1 aa 330-380.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          TBS, pH 7.4, with 50% glycerol, 0.2% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2913444

          Product Specific Information

          Positive Control: NIH/3T3 cell lysate, Raji cell lysate, MCF-7 cell lysate, Daudi cell lysate, mouse liver tissue lysate, rat liver tissue lysate, MCF-7, human small intestine tissue, mouse testis tissue.

          Subcellular Location: Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cell membrane, Early endosome, Early endosome membrane, Cytoplasmic granule, growth cone, neuron projection, axon, synapse.

          Target Information

          Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Alzheimer Disease 3; familial Alzheimer Disease; Minilin; Presenilin-1; presenilin1; Protein S182; PS-1; S182; S182 protein; Senilin 1; unnamed protein product

          View more View less

          Gene Aliases: ACNINV3; AD3; Ad3h; CMD1U; FAD; PS-1; PS1; PSEN1; PSNL1; S182

          View more View less

          UniProt ID: (Human) P49768, (Rat) P97887, (Mouse) P49769

          View more View less

          Entrez Gene ID: (Human) 5663, (Rat) 29192, (Mouse) 19164

          View more View less

          Function(s)
          endopeptidase activity aspartic-type endopeptidase activity calcium channel activity protein binding beta-catenin binding peptidase activity hydrolase activity PDZ domain binding aspartic endopeptidase activity, intramembrane cleaving cadherin binding ATPase binding growth factor receptor binding aspartic protease
          Process(es)
          autophagosome assembly negative regulation of transcription from RNA polymerase II promoter blood vessel development cell fate specification somitogenesis neuron migration positive regulation of receptor recycling heart looping positive regulation of L-glutamate transport hematopoietic progenitor cell differentiation astrocyte activation involved in immune response T cell activation involved in immune response myeloid leukocyte differentiation neural retina development protein glycosylation proteolysis membrane protein ectodomain proteolysis mitochondrial transport cellular calcium ion homeostasis autophagy apoptotic process cellular response to DNA damage stimulus response to oxidative stress cell adhesion Notch signaling pathway Notch receptor processing brain development heart development learning or memory memory post-embryonic development gene expression regulation of gene expression positive regulation of gene expression negative regulation of gene expression regulation of neuron projection development protein transport choline transport synaptic vesicle targeting protein processing cerebellum development cerebral cortex cell migration Cajal-Retzius cell differentiation dorsal/ventral neural tube patterning cerebral cortex development neurogenesis neuron differentiation embryonic limb morphogenesis forebrain development positive regulation of proteasomal ubiquitin-dependent protein catabolic process endoplasmic reticulum calcium ion homeostasis positive regulation of tumor necrosis factor production beta-amyloid formation segmentation intracellular signal transduction locomotion negative regulation of epidermal growth factor receptor signaling pathway positive regulation of protein import into nucleus regulation of phosphorylation amyloid precursor protein metabolic process amyloid precursor protein catabolic process myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of neuron apoptotic process skin morphogenesis positive regulation of glycolytic process positive regulation of transcription, DNA-templated astrocyte activation regulation of synaptic plasticity thymus development neuron development skeletal system morphogenesis brain morphogenesis beta-amyloid metabolic process epithelial cell proliferation negative regulation of axonogenesis synapse organization positive regulation of coagulation T cell receptor signaling pathway sequestering of calcium ion neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis protein maturation regulation of synaptic transmission, glutamatergic calcium ion homeostasis regulation of resting membrane potential regulation of canonical Wnt signaling pathway positive regulation of dendritic spine development neuron cellular homeostasis calcium ion transmembrane transport apoptotic signaling pathway cell-cell adhesion regulation of synaptic vesicle cycle L-glutamate import across plasma membrane regulation of postsynapse organization protein catabolic process at postsynapse cellular response to beta-amyloid negative regulation of core promoter binding positive regulation of amyloid fibril formation neuron projection maintenance negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic signaling pathway activation of MAPKK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation negative regulation of protein kinase activity calcium ion transport negative regulation of epidermal growth factor-activated receptor activity regulation of epidermal growth factor-activated receptor activity L-glutamate transport single organismal cell-cell adhesion eye photoreceptor cell development positive regulation of catalytic activity regulation of protein binding positive regulation of MAP kinase activity cellular protein metabolic process positive regulation of protein kinase activity negative regulation of ubiquitin-protein transferase activity canonical Wnt signaling pathway embryo development anagen
          It has to be done as per old AB suggested Products section.

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