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Invitrogen
GPX1 Polyclonal Antibody
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FIGURE: 1 / 8
GPX1 Antibody (PA5-119990) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Glutathione Peroxidase 1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with GPX1 Polyclonal Antibody (Product # PA5-119990) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution.
Product Details
PA5-119990
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within C-terminalhuman Glutathione peroxidase 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Mouse liver tissue, human liver tissue, 293T, human breast cancer tissue, human kidney tissue, mouse brain tissue, HepG2.
Subcellular Location: Cytoplasm.
Target Information
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular glutathione peroxidase; Glutathione peroxidase 1; GPx-1; GSHPx-1; HGNC:4553; MGC14399; MGC88245; OTTHUMP00000210766; Phospholipid-hydroperoxide glutathione peroxidase GPX1; Selenium-dependent glutathione peroxidase 1; selenoprotein GPX1; unnamed protein product
Gene Aliases: AI195024; AL033363; CGPx; Gpx; GPx-1; GPX1; GPXD; GSHPx-1; GSHPX1
UniProt ID: (Human) P07203, (Mouse) P11352
Entrez Gene ID: (Human) 2876, (Mouse) 14775
response to reactive oxygen species
temperature homeostasis
endothelial cell development
negative regulation of inflammatory response to antigenic stimulus
lipid metabolic process
triglyceride metabolic process
glutathione metabolic process
apoptotic process
response to oxidative stress
sensory perception of sound
intrinsic apoptotic signaling pathway in response to oxidative stress
response to xenobiotic stimulus
response to symbiotic bacterium
response to wounding
response to toxic substance
UV protection
response to hormone
response to glucose
response to selenium ion
response to gamma radiation
myotube differentiation
arachidonic acid metabolic process
lipoxygenase pathway
response to estradiol
response to lipopolysaccharide
response to hydroperoxide
response to vitamin E
regulation of mammary gland epithelial cell proliferation
cellular response to oxidative stress
response to nicotine
regulation of gene expression, epigenetic
vasodilation
response to hydrogen peroxide
hydrogen peroxide catabolic process
skeletal muscle tissue regeneration
blood vessel endothelial cell migration
fat cell differentiation
myoblast differentiation
cell redox homeostasis
fibroblast proliferation
skeletal muscle fiber development
neuron apoptotic process
myoblast proliferation
response to folic acid
interaction with symbiont
positive regulation of protein kinase B signaling
heart contraction
angiogenesis involved in wound healing
regulation of proteasomal protein catabolic process
cellular response to glucose stimulus
negative regulation of release of cytochrome c from mitochondria
cellular oxidant detoxification
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of supramolecular fiber organization
response to lipid hydroperoxide
cell proliferation
protein oxidation
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of neuron apoptotic process
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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