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Invitrogen
PSMA4 Polyclonal Antibody
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FIGURE: 1 / 1
PSMA4 Antibody (PA5-120221) in WB
Western blot analysis of PSMA4 in extracts of various cell lines. Samples were incubated with PSMA4 Polyclonal antibody (Product # PA5-120221) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-120221
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PSMA4 (NP_001096137.1).
Conjugate
Form
Liquid
Concentration
0.77 mg/mL
Amount
77 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: 293T, U-251MG, HeLa, COS-7, PC-12, Jurkat, Mouse liver, Mouse kidney. The target is usually found in the following locations: Cytoplasm, Nucleus, P-body.
Immunogen sequence: MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR NIHKLLDEVF FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK EGEMTLKSAL ALAIKVLNKT MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH EEEEAKAERE KKEKEQKEKD K
Target Information
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-3; Macropain subunit C9; MGC111191; MGC12467; MGC24813; Multicatalytic endopeptidase complex subunit C9; multicatalytic proteinase subunit L; proteasome (prosome, macropain) subunit, alpha type, 4; proteasome alpha 4 subunit; Proteasome component C9; proteasome subunit alpha 4; Proteasome subunit alpha type-4; Proteasome subunit alpha-3; proteasome subunit alpha3; proteasome subunit HC9; Proteasome subunit L; PSMA 4; unnamed protein product
Gene Aliases: C9; HC9; HsT17706; PSC9; PSMA4
UniProt ID: (Human) P25789, (Mouse) Q9R1P0, (Rat) P21670
Entrez Gene ID: (Human) 5685, (Mouse) 26441, (Rat) 29671
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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