Search
Invitrogen
TRIM5 beta Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
TRIM5 beta Antibody (PA5-120537) in WB
Western blot analysis of TRIM5 beta in extracts of various cell lines. Samples were incubated with TRIM5 beta Polyclonal antibody (Product # PA5-120537) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120537
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2).
Conjugate
Form
Liquid
Concentration
1.21 mg/mL
Amount
121 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, SGC-7901, Mouse stomach. The target is usually found in the following locations: Cytoplasm, Nucleus, P-body.
Immunogen sequence: EKLLLFCQED GKVICWLCER SQEHRGHHTF LTEEVAREYQ VKLQAALEML RQKQQEAEEL EADIREEKAS WKTQIQYDKT NVLADFEQLR DILDWEESNE L
Target Information
The protein encoded by this gene is a member of the tripartite motif family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coiled-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiquitinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: RING finger protein 88; RING-type E3 ubiquitin transferase TRIM5; TRIM (RBCC) family member; TRIM5 isoform iota; TRIM5 isoform kappa; tripartite motif protein TRIM5; Tripartite motif-containing protein 5; tripartite motif-containing protein 5 alpha; unnamed protein product
Gene Aliases: EG667823; Gm8833; RGD1304579; RNF88; TRIM5; TRIM5alpha
UniProt ID: (Human) Q9C035
Entrez Gene ID: (Human) 85363, (Mouse) 667823, (Rat) 308906
transcription coactivator activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
protein kinase binding
protein binding, bridging
pattern recognition receptor activity
identical protein binding
protein homodimerization activity
metal ion binding
ubiquitin protein ligase activity
signaling pattern recognition receptor activity
ubiquitin-protein ligase
activation of innate immune response
immune system process
autophagy
regulation of gene expression
protein ubiquitination
regulation of lipopolysaccharide-mediated signaling pathway
regulation of protein localization
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
negative regulation by host of viral release from host cell
innate immune response
positive regulation of transcription, DNA-templated
regulation of viral entry into host cell
negative regulation of viral entry into host cell
positive regulation of NF-kappaB transcription factor activity
defense response to virus
protein K63-linked ubiquitination
regulation of primary metabolic process
pattern recognition receptor signaling pathway
positive regulation of sequence-specific DNA binding transcription factor activity
protein trimerization
negative regulation of viral release from host cell
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support