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Invitrogen
BAP1 Polyclonal Antibody
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FIGURE: 1 / 7
BAP1 Antibody (PA5-12061) in ICC/IF
Immunocytochemistry analysis of BAP1 in HeLa cells. Samples were incubated with BAP1 polyclonal antibody (Product # PA5-12061) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-Rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing Nucleus and Weak Cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
Product Details
PA5-12061
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 36-66 amino acids of human BAP1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Protein A, Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, rat and zebrafish based on sequence homology.
Target Information
BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4. 8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21. 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BRCA1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; DKFZp686N04275; FLJ35406; FLJ37180; KIAA0272; medulloblastoma antigen; Ubiquitin C-terminal hydrolase X4; ubiquitin carboxy-terminal hydrolase L2; ubiquitin carboxyl-terminal hydrolase; Ubiquitin carboxyl-terminal hydrolase BAP1; UCH-X4; unnamed protein product
Gene Aliases: 2300006C11Rik; AA989761; AW553466; BAP1; HUCEP-13; hucep-6; KIAA0272; KURIS; mKIAA0272; TPDS1; UBM2; uch-x4; UCHL2; UVM2
UniProt ID: (Human) Q92560, (Rat) D3ZHS6, (Mouse) Q99PU7
Entrez Gene ID: (Human) 8314, (Rat) 306257, (Mouse) 104416
mitotic cell cycle
regulation of cell growth
in utero embryonic development
tissue homeostasis
thrombocyte differentiation
chromatin organization
proteolysis
ubiquitin-dependent protein catabolic process
cell proliferation
negative regulation of cell proliferation
gene expression
regulation of gene expression
protein deubiquitination
cell differentiation
erythrocyte differentiation
neutrophil differentiation
granulocyte differentiation
heterochromatin assembly
myeloid cell apoptotic process
monoubiquitinated protein deubiquitination
common myeloid progenitor cell proliferation
protein modification process
platelet morphogenesis
erythrocyte maturation
nucleate erythrocyte differentiation
negative regulation of transcription, DNA-templated
regulation of inflammatory response
regulation of cell cycle
hematopoietic stem cell homeostasis
macrophage homeostasis
neuron cellular homeostasis
leukocyte proliferation
protein K48-linked deubiquitination
regulation of cytokine production involved in inflammatory response
positive regulation of protein targeting to mitochondrion
monoubiquitinated histone H2A deubiquitination
chromatin modification
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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