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Invitrogen
EphA1 Polyclonal Antibody
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FIGURE: 1 / 1
EphA1 Antibody (PA5-121089) in WB
Western blot analysis of EphA1 in extracts of various cell lines. Samples were incubated with EphA1 Polyclonal antibody (Product # PA5-121089) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-121089
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human EPHA1 (NP_005223.4).
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Amount
47 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: BT-474, HT-29, Mouse liver, Mouse lung, Mouse kidney. The target is usually found in the following locations: Cell membrane, Single-pass type I membrane protein.
Immunogen sequence: MERRWPLGLG LVLLLCAPLP PGARAKEVTL MDTSKAQGEL GWLLDPPKDG WSEQQQILNG TPLYMYQDCP MQGRRDTDHW LRSNWIYRGE EASRVHVELQ FTVRDCKSFP GGAGPLGCKE TFNLLYMESD QDVGIQLRRP LFQKVTTVAA DQSFTIRDLV SGSVKLNVER CSLGRLTRRG LYLAFHNPGA CVALVSVRVF YQRCPETLNG LAQFPDTLPG PAGLVEVAGT
Target Information
EphA1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA1 is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Embryonic stem cell kinase; EPH tyrosine kinase; EPH tyrosine kinase 1; Ephrin type-A receptor 1; erythropoietin-producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; hEpha1; mEpha1; MGC163163; oncogene EPH; tyrosine kinase receptor precursor (put.); putative; Tyrosine-protein kinase receptor EPH; Tyrosine-protein kinase receptor ESK; unnamed protein product
Gene Aliases: 5730453L17Rik; AL033318; EPH; EPHA1; EPHT; EPHT1; Esk
UniProt ID: (Human) P21709, (Mouse) Q60750
Entrez Gene ID: (Human) 2041, (Mouse) 13835
nucleotide binding
fibronectin binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
transmembrane-ephrin receptor activity
ATP binding
kinase activity
transferase activity
protein kinase binding
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
angiogenesis
positive regulation of cell-matrix adhesion
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of cell adhesion
positive regulation of cell migration
negative regulation of cell migration
substrate adhesion-dependent cell spreading
positive regulation of angiogenesis
ephrin receptor signaling pathway
positive regulation of stress fiber assembly
protein phosphorylation
negative regulation of protein kinase activity
cell surface receptor signaling pathway
positive regulation of cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
regulation of GTPase activity
protein autophosphorylation
activation of GTPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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