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Invitrogen
PIST Polyclonal Antibody
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FIGURE: 1 / 1
PIST Antibody (PA5-121111) in WB
Western blot analysis of PIST in extracts of various cell lines. Samples were incubated with PIST Polyclonal antibody (Product # PA5-121111) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121111
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human PIST/PIST/GOPC (NP_0010174081)
Conjugate
Form
Liquid
Concentration
1.89 mg/mL
Amount
189 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, Mouse kidney, Mouse liver, Mouse brain, Rat brain. The target is usually found in the following locations: Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein, dendrite, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network membrane.
Immunogen sequence: ARLAAKYLDK ELAGRVQQIQ LLGRDMKGPA HDKLWNQLEA EIHLHRHKTV IRACRGRNDL KRPMQAPPGH DQDSLKKSQG VGPIRKVLLL KEDHEGLGIS ITGGKEHGVP ILISEIHPGQ PADRCGGLHV GDAILAVNGV NLRDTKHKEA VTILSQQRGE IEFEVVYVAP EVDSDDENVE YEDESGHRYR LYLDELEGGG NPGASCKDTS GEIKVLQGFN KKAVTDTHEN GDLGTASETP LDDGASKLDD LHTLYHKKSY
Target Information
PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CFTR-associated ligand; dJ94G16.2 PIST; FIG; PDZ domain-containing Golgi protein; Fused in glioblastoma; golgi-associated PDZ and coiled-coil motif containing protein; Golgi-associated PDZ and coiled-coil motif-containing protein; PDZ domain-containing protein; PDZ domain-containing protein PIST; PDZ protein interacting specifically with TC10; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10; PIST
Gene Aliases: 2210402P09Rik; AI844555; CAL; dJ94G16.2; FIG; GOPC; GOPC1; PIST
UniProt ID: (Human) Q9HD26, (Mouse) Q8BH60
Entrez Gene ID: (Human) 57120, (Mouse) 94221, (Rat) 309774
ER to Golgi vesicle-mediated transport
Golgi to plasma membrane transport
negative regulation of anion channel activity
protein transport
apical protein localization
negative regulation of protein localization to cell surface
transport
autophagy
spermatid nucleus differentiation
regulation of catalytic activity
protein homooligomerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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