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Invitrogen
Claudin 2 Polyclonal Antibody
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FIGURE: 1 / 4
Claudin 2 Antibody (PA5-13334) in IHC (P)
Immunohistochemistry analysis of Claudin 2 in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with Claudin 2 polyclonal antibody (Product # PA5-13334) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Details
PA5-13334
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 135-164 amino acids from the N-terminal region of human Claudin 2 (CLDN2)
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Amount
180 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine and mouse based on sequence homology.
Target Information
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Confinement of, for example, the glucose symport to apical surfaces allows glucose to be transported vectorially from the lumen, through the cell, and into the bloodstream. Several peripheral membrane proteins are associated with tight junctions including ZO-1, ZO-2, ZO-3 (members of membrane-associated guanylate-kinase family), cingulin, the 7H6 antigen, Rab-3b, symplekin. While their precise functions are not known, roles for these proteins have been suggested in tight junction assembly and maintenance; signal transduction; and the regulation of tight junction permeability. Furthermore, a growing body of evidence suggests that actin filaments play a major role in regulating tight junction permeability. Until recently, the only transmembrane protein known to be associated with tight junctions was occludin, an ~65 kDa protein with four transmembrane domains. Despite widespread expectation, a critical structural role for occludin in TJ strands was ruled out by the observation of apparently normal tight junctions formed between cells disrupted at both occludin alleles. Fortunately, a closer examination of isolated tight junctions uncovered two related ~22 kDa, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions. Though inconclusive, these findings suggest that claudin-1 and -2 are major structural components of TJ strands and that occludin plays some other accessory role. Excitement in the tight junction field continues to rise following the recent discovery of claudins -3, -4, -5, -6, -7, and -8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. Claudin-1 and claudin-2 connect to the actin cytoskeleton through ZO-1; Claudin-2 functions as a paracellular channel with cation (Na+) selectivity at tight junctions. The expression of claudin-2 is restricted to the liver and kidney, with small amounts also found in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Claudin-2; Claudin2; CLD2; SP82; tight junction-associated protein; unnamed protein product
Gene Aliases: claudin-2; CLDN2; OAZON; PSEC0059; SP82; UNQ705/PRO1356
UniProt ID: (Human) P57739
Entrez Gene ID: (Human) 9075
innate immune response in mucosa
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
bicellular tight junction assembly
cell-cell adhesion
regulation of bile acid secretion
paracellular transport
regulation of intestinal D-glucose absorption
regulation of intestinal lipid absorption
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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