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Invitrogen
GNB1 Polyclonal Antibody
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FIGURE: 1 / 5
GNB1 Antibody (PA5-141030) in ICC/IF
Immunocytochemistry analysis of GNB1 in SiHa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in GNB1 Polyclonal antibody (Product # PA5-141030) using a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451; followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibod... View More
Product Details
PA5-141030
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human GNB1 aa 100-340.
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Amount
102 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta subunit, signal-transducing proteins GS/GI; G protein beta 1; gbb1; GNB1; guanine nucleotide binding protein (G protein), beta 1; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide binding protein, beta 1; Guanine nucleotide-binding protein beta 1; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein, beta-1 subunit; heterotrimeric guanine nucleotide-binding protein 2A; T beta; testicular tissue protein Li 72; Transducin beta chain 1; transducin beta-1 subunit; unnamed protein product
Gene Aliases: AA409223; C77571; Gnb-1; GNB1; HG2A; MDS; MRD42
UniProt ID: (Human) P62873, (Mouse) P62874, (Rat) P54311
Entrez Gene ID: (Human) 2782, (Mouse) 14688, (Rat) 24400
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating dopamine receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
G-protein coupled acetylcholine receptor signaling pathway
Ras protein signal transduction
cell proliferation
sensory perception of taste
retina development in camera-type eye
cellular response to prostaglandin E stimulus
cellular response to catecholamine stimulus
positive regulation of cytosolic calcium ion concentration
phototransduction, visible light
cardiac muscle cell apoptotic process
cellular response to hypoxia
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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