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Invitrogen
USP8 Polyclonal Antibody
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FIGURE: 1 / 3
USP8 Antibody (PA5-141050) in IHC (P)
Immunohistochemistry analysis of USP8 in paraffin-embedded human colon carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with USP8 Polyclonal antibody (Product # PA5-141050) using a dilution of 1:1,000 for 1 hour at... View More
Product Details
PA5-141050
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human USP8 aa 1-250.
Conjugate
Form
Liquid
Concentration
2.3 mg/mL
Amount
230 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
USP8 is a ubiquitin specific protease that plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 is involved in cell proliferation, and probably regulates the stability of STAM2 and RASGRF1. USP8 may regulate T-cell energy mediated by RNF128 via the formation of a complex containing RNF128 and STAM2. As revealed by structure/function studies, USP8 forms a ternary complex with RNF128 and OTUB1, and interacts with the SH3 domain of STAM2 and RASGRF1. Expression of USP8 is induced Upon growth stimulation in starved human fibroblasts, and expression decreases in response to growth arrest induced by cell-cell contact.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Deubiquitinating enzyme 8; hUBPy; KIAA0055; mUBPy; putative deubiquitinating enzyme; Ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin isopeptidase Y; ubiquitin specific protease 8; Ubiquitin thioesterase 8; ubiquitin thiolesterase 8; Ubiquitin-specific-processing protease 8; unnamed protein product
Gene Aliases: AI574262; AW557536; HumORF8; KIAA0055; mKIAA0055; PITA4; SPG59; UBPY; USP8
UniProt ID: (Human) P40818, (Mouse) Q80U87
Entrez Gene ID: (Human) 9101, (Rat) 296121, (Mouse) 84092
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
SH3 domain binding
cadherin binding
Lys63-specific deubiquitinase activity
Lys48-specific deubiquitinase activity
thiol-dependent ubiquitinyl hydrolase activity
cysteine protease
mitotic cytokinesis
proteolysis
endosome organization
Ras protein signal transduction
protein deubiquitination
regulation of protein stability
regulation of protein localization
protein K63-linked deubiquitination
protein K48-linked deubiquitination
cellular response to dexamethasone stimulus
positive regulation of canonical Wnt signaling pathway
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
negative regulation of lysosomal protein catabolic process
positive regulation of amyloid fibril formation
cellular response to nerve growth factor stimulus
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
ubiquitin-dependent protein catabolic process
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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