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Invitrogen

AIF Polyclonal Antibody

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AIF Polyclonal Antibody

Product Details

PA5-143222

Applications
Tested Dilution
Publications

Western Blot (WB)

0.01-0.1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence C-NEVAKLFNIHED.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2933861

Product Specific Information

Additional Information: This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

Target Information

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AG1; AGR1; apoptogenic protein; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; auditory neuropathy, X-linked recessive 1; cell death factor; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; harlequin; hTLP19; MGC111425; mitochondrial apoptosis-inducing factor 1; NADH oxidase; pcd8; PDCD8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; unnamed protein product; UNQ713/PRO1376

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Gene Aliases: AIF; AIFM1; AIFsh2; AUNX1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8; SEMDHL

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UniProt ID: (Human) O95831, (Mouse) Q9Z0X1, (Rat) Q9JM53

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Entrez Gene ID: (Human) 9131, (Pig) 100524062, (Mouse) 26926, (Rat) 83533

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Function(s)
DNA binding NADH dehydrogenase activity protein binding NAD(P)H oxidase activity oxidoreductase activity oxidoreductase activity, acting on NAD(P)H protein dimerization activity FAD binding poly-ADP-D-ribose binding C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity aldo-keto reductase (NADP) activity electron-transferring-flavoprotein dehydrogenase activity isocitrate dehydrogenase activity mevaldate reductase activity gluconate dehydrogenase activity steroid dehydrogenase activity epoxide dehydrogenase activity 5-exo-hydroxycamphor dehydrogenase activity 2-hydroxytetrahydrofuran dehydrogenase activity acetoin dehydrogenase activity phenylcoumaran benzylic ether reductase activity D-xylose:NADP reductase activity L-arabinose:NADP reductase activity D-arabinitol dehydrogenase, D-ribulose forming (NADP+) activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor steroid dehydrogenase activity, acting on the CH-CH group of donors (R)-(-)-1,2,3,4-tetrahydronaphthol dehydrogenase activity 3-hydroxymenthone dehydrogenase activity very long-chain-3-hydroxyacyl-CoA dehydrogenase activity dihydrotestosterone 17-beta-dehydrogenase activity (R)-2-hydroxyisocaproate dehydrogenase activity L-arabinose 1-dehydrogenase (NADP+) activity L-xylulose reductase (NAD+) activity 3-ketoglucose-reductase activity flavin adenine dinucleotide binding (R)-2-hydroxyglutarate dehydrogenase activity D-arabinitol dehydrogenase, D-xylulose forming (NADP+) activity oxidoreductase
Process(es)
response to ischemia apoptotic process response to toxic substance neuron differentiation mitochondrial respiratory chain complex assembly positive regulation of apoptotic process positive regulation of programmed cell death positive regulation of neuron apoptotic process protein import into mitochondrial intermembrane space positive regulation of necroptotic process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to estradiol stimulus cellular response to hypoxia cellular response to nitric oxide mitochondrial disulfide relay system response to L-glutamate cellular response to aldosterone activation of cysteine-type endopeptidase activity involved in apoptotic process response to oxidative stress apoptotic mitochondrial changes positive regulation of cell death mitochondrial respiratory chain complex I assembly cell redox homeostasis neuron apoptotic process oxidation-reduction process regulation of apoptotic DNA fragmentation
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