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Invitrogen
Phospho-DOK1/DOK2 (Tyr362, Tyr337) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-DOK1/DOK2 (Tyr362, Tyr337) Antibody (PA5-143681) in WB
Western blot of Phospho-DOK1/DOK2 (Tyr362, Tyr337) in Jurkat cells stimulated with calyculin A (100 nM, 30 min.) (lanes 1-6) followed by lambda phosphatase (lanes 2 & 6) or alkaline phosphatase (lane 4) treatment. The blots were probed with anti-Dok1 (lanes 1 & 2), Phospho-DOK1/DOK2 (Tyr362, Tyr337) polyclonal antibody (Product # PA5-143681) (lanes 3 & 4), and anti-Dok1 (Ser450) (lanes 5 & 6).
Product Details
PA5-143681
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-Dok1 (Tyr-362) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding tyrosine 362 in human Dok1. This sequence is conserved in Dok1 from rat and mouse (Tyr-361), and has high homology to Dok2 (Tyr-337). The site is not conserved in other Dok family members.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment. Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified.
Target Information
Doks are a family of adaptor proteins that include six Dok proteins (Dok1 to Dok6), which have an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras-GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IkappaB Kinase beta phosphorylates several serine sites including Ser-450 in vitro, and TNFalpha, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Docking protein 1; Downstream of tyrosine kinase 1; downstream of tyrosine kinase-1; p62(dok); p62DOK; pp62; similar to GAP binding protein p62do; unnamed protein product
Gene Aliases: AW557123; Dok; Dok-1; DOK1; P62DOK; pp62
UniProt ID: (Human) Q99704, (Rat) Q4QQV2, (Mouse) P97465
Entrez Gene ID: (Human) 1796, (Rat) 312477, (Mouse) 13448
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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