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Invitrogen
MBNL1 Polyclonal Antibody
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FIGURE: 1 / 10
MBNL1 Antibody (PA5-143882) in ICC/IF
Immunocytochemistry analysis of MBNL1 in A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in MBNL1 Polyclonal antibody (Product # PA5-143882) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-143882
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MBNL1 recombinant protein (Position: Q89-M388).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, monkey COS-7 whole cell, human 293T whole cell, human Hela whole cell, human MOLT-4 whole cell, rat heart tissue. IHC: human thyroid cancer tissue, human colorectal adenocarcinoma tissue, human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human ovarian serous adenocarcinoma tissue, mouse testis tissue, rat testis tissue. ICC/IF: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 35 kDa; CUG triplet repeat expansion dsRNA-binding protein; C3H type zinc finger protein; C3H zinc finger dsRNA-binding protein; C3H Zn finger dsRNA binding protein; DKFZp686P06174; EXP35; EXP40; EXP42; KIAA0428; muscleblind-like; muscleblind-like 1; Muscleblind-like protein 1; Triplet-expansion RNA-binding protein; unnamed protein product
Gene Aliases: EXP; KIAA0428; MBNL; MBNL1; mKIAA0428
UniProt ID: (Human) Q9NR56, (Rat) A0A8I6B1J2, (Mouse) Q9JKP5
Entrez Gene ID: (Human) 4154, (Rat) 282635, (Mouse) 56758
in utero embryonic development
mRNA processing
nervous system development
RNA splicing
embryonic limb morphogenesis
regulation of RNA splicing
myoblast differentiation
alternative mRNA splicing, via spliceosome
regulation of alternative mRNA splicing, via spliceosome
mRNA splice site selection
skeletal muscle tissue development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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