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Invitrogen
GATA5 Polyclonal Antibody
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FIGURE: 1 / 1
GATA5 Antibody (PA5-144085) in WB
Western blot analysis of GATA5 using GATA5 antibody (Product # PA5-144085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human 293T whole cell lysates; Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was... View More
Product Details
PA5-144085
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human GATA5 recombinant protein (Position: M1-A397).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5.
Positive Control - WB: human 293T whole cell, human HepG2 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
GATA binding factor 5 is a transcription regulator involved in embryonic development. In addition, the protein encoded by this gene contains two GATA-type zinc fingers and is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha). This interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. GATA-5 may be involved in the establishment of cardiac smooth muscle cell diversity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GATA binding factor-5; GATA-binding factor 5; Transcription factor GATA-5
Gene Aliases: bB379O24.1; CHTD5; GATA5; GATAS
UniProt ID: (Human) Q9BWX5
Entrez Gene ID: (Human) 140628
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
heart induction
aortic valve morphogenesis
endocardial cushion fusion
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
heart development
anatomical structure morphogenesis
tissue development
negative regulation of cardiac muscle hypertrophy
positive regulation of gene expression
negative regulation of gene expression
cell differentiation
cell fate commitment
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cardiac muscle tissue development
intestinal epithelial cell differentiation
positive regulation of cardiac endothelial to mesenchymal transition
cellular response to hydrogen peroxide
cellular response to nitric oxide
cellular response to BMP stimulus
positive regulation of apoptotic DNA fragmentation
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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