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Invitrogen
ADAM17 Polyclonal Antibody
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FIGURE: 1 / 6
ADAM17 Antibody (PA5-17079) in ICC/IF
Immunofluorescence analysis of ADAM17 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ADAM17 Polyclonal Antibody (Product # PA5-17079) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
Product Details
PA5-17079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids surrounding Val200 of human TACE
Conjugate
Form
Liquid
Concentration
90 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ADAM17 (disintegrin and metalloproteinase domain-containing protein 17) cleaves the membrane bound precusor of TNF-alpha to its mature soluble form. ADAM 17 is responsbile for the proteolytical release of soluble JAM3 from endothelial cells surface and the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. It acts as an activator of the Notch pathway by mediating cleavage of Notch and generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Mutations in the gene can result in inflammatory skin and bowel disease, neonatal 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a disintegrin and metalloproteinase; a disintegrin and metalloproteinase 17; ADAM; ADAM 17; ADAM metallopeptidase domain 18; ADAMs; cartilage snake venom-like protease; CD156b; CD156b antigen; Disintegrin and metalloproteinase domain-containing protein 17; metalloendopeptidases; Snake venom-like protease; TNF-alpha convertase; TNF-alpha convertase enzyme; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme; tumor overexpression; unnamed protein product
Gene Aliases: ADAM17; ADAM18; CD156B; CSVP; NISBD; NISBD1; TACE
UniProt ID: (Human) P78536
Entrez Gene ID: (Human) 6868
endopeptidase activity
metalloendopeptidase activity
Notch binding
interleukin-6 receptor binding
integrin binding
protein binding
peptidase activity
metallopeptidase activity
hydrolase activity
SH3 domain binding
cytokine binding
PDZ domain binding
tumor necrosis factor binding
metal ion binding
metallodipeptidase activity
metalloendopeptidase activity involved in amyloid precursor protein catabolic process
response to hypoxia
neutrophil mediated immunity
germinal center formation
production of molecular mediator involved in inflammatory response
positive regulation of leukocyte chemotaxis
proteolysis
membrane protein ectodomain proteolysis
cell adhesion
epidermal growth factor receptor signaling pathway
Notch signaling pathway
Notch receptor processing
positive regulation of cell proliferation
response to xenobiotic stimulus
positive regulation of signal transduction
positive regulation of T cell chemotaxis
negative regulation of neuron projection development
protein processing
signal release
B cell differentiation
positive regulation of cell growth
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
response to lipopolysaccharide
positive regulation of chemokine production
positive regulation of tumor necrosis factor production
regulation of mast cell apoptotic process
T cell differentiation in thymus
cell adhesion mediated by integrin
wound healing, spreading of epidermal cells
amyloid precursor protein catabolic process
protein kinase B signaling
positive regulation of blood vessel endothelial cell migration
positive regulation of epidermal growth factor receptor signaling pathway
spleen development
regulation of axon regeneration
cell motility
defense response to Gram-positive bacterium
cellular response to high density lipoprotein particle stimulus
commissural neuron axon guidance
negative regulation of cold-induced thermogenesis
cytokine precursor processing
signaling receptor ligand precursor processing
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of vascular endothelial cell proliferation
regulation of neuron migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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