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Invitrogen
IRAK1 Polyclonal Antibody
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FIGURE: 1 / 12
IRAK1 Antibody (PA5-17490) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), HT-1080 (Lane 4), COLO 205 (Lane 5), MDA-MB-231 (Lane 6), COS-7 (Lane 7) and THP-1 (Lane 8). The blot was probed with Anti-IRAK1 Polyclonal Antibody (Product # PA5-17490, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). An 80kDa band corresponding to IRAK1 was observed across all ... View More
Product Details
PA5-17490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding glycine 696 of human IRAK1
Conjugate
Form
Liquid
Concentration
26.4 µg/mL
Amount
2.64 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hIRAK1c; protein kinase; inactive protein kinase; signaling molecule; Interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog
Gene Aliases: IRAK; IRAK1; pelle
UniProt ID: (Human) P51617
Entrez Gene ID: (Human) 3654
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
heat shock protein binding
identical protein binding
protein homodimerization activity
protein heterodimerization activity
protein serine kinase activity
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
immune system process
MyD88-dependent toll-like receptor signaling pathway
regulation of transcription, DNA-templated
signal transduction
I-kappaB kinase/NF-kappaB signaling
JNK cascade
Toll signaling pathway
positive regulation of macromolecule metabolic process
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
cellular response to heat
intracellular signal transduction
interleukin-33-mediated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
protein autophosphorylation
positive regulation of smooth muscle cell proliferation
positive regulation of NF-kappaB transcription factor activity
type I interferon signaling pathway
interleukin-1-mediated signaling pathway
response to interleukin-1
cellular response to lipopolysaccharide
cellular response to hypoxia
positive regulation of intracellular signal transduction
positive regulation of leukocyte adhesion to vascular endothelial cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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