Invitrogen

GCN2 Polyclonal Antibody

Name: GCN2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-17523

Advanced Verification

1 Published Figure

2 References

View all (23) GCN2 antibodies

Datasheet

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Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

FIGURE: 1 / 3

GCN2 Antibody (PA5-17523) in WB

Western blot analysis of GCN2 in extracts from HT1376 cells that were untreated, treated with UV light (50 mJ/cm2 for 30 minutes), or subjected to nocodazole (50 ng/mL, 24hrs), using GCN2 polyclonal antibody (Product # PA5-17523).

Product Image Gallery

GCN2 Antibody — Figure 4. GCN2-peIF2a-ATF4 signaling mediates the upregulation of WARS in response to Trp depletion. (A) GCN2 mRNA expression measured by qRT-PCR in LN229 cells after knockdown of GCN2 by siRNA cultured in the absence and presence of 78 uM Trp for 24 h. (B) WARS mRNA expression measured by qRT-PCR and (C) WARS and GCN2 protein expression detected by Western blot under the conditions described in (A). GAPDH served as loading control. (D) WARS2 mRNA expression measured by qRT-PCR under the conditions described in (A). (E) Gcn2 mRNA expression in wildtype (wt) and Gcn2 -/- MEFs cultured in the absence and presence of 78 uM Trp for 24 h. (F) Wars mRNA expression measured by qRT-PCR and (G) WARS and GCN2 protein expression detected by Western blot under the conditions described in (E). Tubulin served as loading control. (H) Wars2 mRNA expression measured by qRT-PCR under the conditions described in (E). (I) WARS mRNA expression measured by qRT-PCR in full DMEM medium (DMEM), in amino acid-free DMEM supplemented with all amino acids (+ all AA) and in amino acid-free DMEM supplemented with all but each of the essential amino acids (EAA). (J) peIF2a and eIF2a detected by Western blot in LN229 cells cultured in the presence (+) or absence (-) of 78 uM Trp for 24 h. (K) Wars mRNA expression measured by qRT-PCR in MEFs that express wildtype Eif2a (SS) or an Eif2a version, in which serine 51 is mutated to an alanine and cannot be phosphorylated by Gcn2 (AA). MEFs were cultured for 24 h i {KD}

Immunogen image

Immunogen Image GCN2 Polyclonal Antibody

Product Details

PA5-17523

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to sequence near the amino terminus of human GCN2

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

16 µg/mL

1.6 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983800

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

EIF2AK4 (GCN2) is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor-2 in response to low amino acid availability. It plays a role as an activator of the integrated stress response required for adapatation to amino acid starvation. Mutations in the gene lead to pulmonary vennocclusive disease 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2; mGCN2; unnamed protein product

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Gene Aliases: 2610011M03; EIF2AK4; GCN2; KIAA1338; MGCN2; PVOD2

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UniProt ID: (Human) Q9P2K8, (Mouse) Q9QZ05

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Entrez Gene ID: (Human) 440275, (Mouse) 27103, (Rat) 114859

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Function(s)

tRNA binding nucleotide binding RNA binding protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity ATP binding kinase activity transferase activity protein serine kinase activity transferase activity, transferring phosphorus-containing groups eukaryotic initiation factor eIF2 binding aminoacyl-tRNA synthetase non-receptor serine/threonine protein kinase translational protein

Process(es)

DNA damage checkpoint positive regulation of defense response to virus by host adaptive immune response T cell activation involved in immune response immune system process positive regulation of adaptive immune response translational initiation regulation of translation regulation of translational initiation protein phosphorylation nervous system development learning long-term memory regulation of translational initiation by eIF2 alpha phosphorylation viral translation negative regulation of translational initiation in response to stress negative regulation of CREB transcription factor activity cellular response to amino acid starvation cellular response to UV response to endoplasmic reticulum stress negative regulation by host of viral genome replication negative regulation of neuron differentiation negative regulation of translational initiation protein autophosphorylation defense response to virus regulation of cell cycle regulation of feeding behavior cellular response to cold positive regulation of translational initiation in response to starvation GCN2-mediated signaling positive regulation of long-term synaptic potentiation neuron projection extension cellular response to starvation positive regulation of gene expression phosphorylation negative regulation of translation endoplasmic reticulum unfolded protein response regulation of translational initiation in response to stress

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GCN2 Polyclonal Antibody

GCN2 Antibody (PA5-17523) in WB

Product Details

PA5-17523

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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