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Invitrogen
PAX5 Polyclonal Antibody
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FIGURE: 1 / 5
PAX5 Antibody (PA5-18079) in ICC/IF
Immunocytochemistry analysis of PAX5 using PAX5 Polyclonal Antibody (Product # PA5-18079) in paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (DLEKNYPTPRTSR) corresponding to the N-terminus amino acids of PAX5 (aa 2-14).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; BSAP; transcription factor; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax-5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5
Gene Aliases: ALL3; BSAP; EBB-1; KLP; PAX-5; PAX5; RGD1565236
UniProt ID: (Human) Q02548, (Mouse) Q02650
Entrez Gene ID: (Human) 5079, (Rat) 500453, (Mouse) 18507
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
skeletal muscle cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of histone H3-K9 methylation
multicellular organism development
spermatogenesis
nervous system development
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
cell differentiation
adult behavior
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
embryonic cranial skeleton morphogenesis
regulation of transcription from RNA polymerase II promoter
sensory organ development
B cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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