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Invitrogen
Blimp-1 Polyclonal Antibody
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FIGURE: 1 / 7
Blimp-1 Antibody (PA5-19216) in ICC/IF
Immunocytochemistry analysis of Blimp-1 using Blimp-1 Polyclonal Antibody (Product # PA5-19216) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-19216
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (DISDNADRLEDVED) corresponding to the internal amino acids of PRDM1 (aa 734-748).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1,000.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1 with ZNF domain isoform 2; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-BF1 alpha; PRDI-BF1 beta; PRDI-binding factor-1; RP1-134E15.1; unnamed protein product
Gene Aliases: BLIMP-1; BLIMP1; PRDI-BF1; PRDM1
UniProt ID: (Human) O75626
Entrez Gene ID: (Human) 639
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
methyltransferase activity
zinc ion binding
transferase activity
metal ion binding
histone methyltransferase binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
morphogenesis of a branching structure
kidney development
embryonic placenta development
maternal placenta development
adaptive immune response
immune system process
heart valve development
cardiac septum development
ventricular septum development
regulation of transcription from RNA polymerase II promoter
germ cell development
post-embryonic development
gene expression
positive regulation of gene expression
negative regulation of gene expression
methylation
regulation of natural killer cell differentiation
regulation of extrathymic T cell differentiation
aorta development
regulation of cell proliferation
eye photoreceptor cell development
innate immune response
cell fate commitment
artery morphogenesis
cellular developmental process
regulation of NK T cell differentiation
retinal bipolar neuron differentiation
intestinal epithelial cell development
trophoblast giant cell differentiation
coronary vasculature development
sebum secreting cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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