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Invitrogen
POU3F3 Polyclonal Antibody
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FIGURE: 1 / 3
POU3F3 Antibody (PA5-19305) in ICC/IF
Immunocytochemistry analysis of POU3F3 using POU3F3 Polyclonal Antibody (Product # PA5-19305) in paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-19305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (HMLSHAHQWVTAL) corresponding to the internal amino acids of POU3F3 (aa 88-101).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation, Antigen affinity chromatography
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with human based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.
Target Information
POU3F3 is a member of the class III POU family of transcription factors that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 8-Oct; Brain-1; Brain-specific homeobox/POU domain protein 1; Oct-8; Octamer-binding protein 8; Octamer-binding transcription factor 8; OTF-8; POU domain, class 3, transcription factor 3
Gene Aliases: Brn-1; Brn1; Otf8; Pou3f3; Skin1
UniProt ID: (Mouse) P31361
Entrez Gene ID: (Mouse) 18993
negative regulation of transcription from RNA polymerase II promoter
kidney development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
nervous system development
brain development
positive regulation of cell proliferation
positive regulation of gene expression
cerebral cortex radially oriented cell migration
forebrain ventricular zone progenitor cell division
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
metanephric ascending thin limb development
metanephric macula densa development
metanephric thick ascending limb development
metanephric loop of Henle development
metanephric DCT cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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