Search

  • Contáctenos
  • Orden Rápida

Invitrogen

TSC1 Polyclonal Antibody

2 Published Figures
5 References
View all (29) TSC1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

PA5-20131

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid synthetic peptide from the middle region of human TSC1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154814

Product Specific Information

This antibody recognizes both isoforms of TSC1.

A suggested positive control is EL4 cell lysate.

PA5-20131 can be used with blocking peptide PEP-0249.

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Hamartin; HGNC:12362; KIAA0243; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; unnamed protein product

View more View less

Gene Aliases: KIAA0243; LAM; TSC; TSC1

View more View less

UniProt ID: (Human) Q92574, (Rat) Q9Z136, (Mouse) Q9EP53

View more View less

Entrez Gene ID: (Human) 7248, (Rat) 60445, (Mouse) 64930

View more View less

Function(s)
protein binding Hsp70 protein binding ATPase inhibitor activity protein binding involved in protein folding chaperone binding Hsp90 protein binding GTPase regulator activity GTPase activating protein binding protein N-terminus binding
Process(es)
kidney development neural tube closure regulation of cell-matrix adhesion adaptive immune response protein folding potassium ion transport cell-matrix adhesion nervous system development cell proliferation negative regulation of cell proliferation associative learning adult locomotory behavior cellular response to starvation negative regulation of macroautophagy hippocampus development cerebral cortex development cell projection organization negative regulation of TOR signaling negative regulation of ATPase activity response to insulin cellular response to decreased oxygen levels TORC1 signaling myelination memory T cell differentiation negative regulation of cell size glucose import synapse organization protein stabilization regulation of stress fiber assembly regulation of cell cycle positive regulation of focal adhesion assembly cardiac muscle cell differentiation activation of GTPase activity negative regulation of TORC1 signaling positive regulation of TORC1 signaling rRNA export from nucleus regulation of translation negative regulation of translation regulation of actin cytoskeleton organization regulation of phosphoprotein phosphatase activity regulation of protein kinase activity negative regulation of insulin receptor signaling pathway protein heterooligomerization regulation of focal adhesion assembly embryo development regulation of GTPase activity
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support