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Invitrogen
BRCC3 Polyclonal Antibody
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Product Details
PA5-20426
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
An 18 amino acid peptide from near the amino terminus of human BRCC36.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is MCF7 cell lysate.
PA5-20426 can be used with blocking peptide PEP-0543.
Target Information
Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6.1A protein; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; C6.1A; Lys-63-specific deubiquitinase BRCC36; RP11-143H17.2; unnamed protein product
Gene Aliases: BRCC3; BRCC36; C6.1A; CXorf53
UniProt ID: (Human) P46736, (Rat) B2RYM5, (Mouse) P46737
Entrez Gene ID: (Human) 79184, (Rat) 316794, (Mouse) 210766
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
metallopeptidase activity
hydrolase activity
enzyme regulator activity
polyubiquitin binding
metal ion binding
Lys63-specific deubiquitinase activity
metal-dependent deubiquitinase activity
pyroglutamyl-peptidase activity
translation initiation factor
DNA repair
regulation of DNA repair
double-strand break repair
chromatin organization
proteolysis
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
response to X-ray
response to ionizing radiation
protein deubiquitination
NLRP3 inflammasome complex assembly
mitotic G2/M transition checkpoint
positive regulation of DNA repair
cell division
protein K63-linked deubiquitination
cellular response to ionizing radiation
DNA repair-dependent chromatin remodeling
positive regulation of NLRP3 inflammasome complex assembly
regulation of DNA damage checkpoint
regulation of catalytic activity
histone H2A K63-linked deubiquitination
chromatin modification
G2 DNA damage checkpoint
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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