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Invitrogen
FOXO4 Polyclonal Antibody
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FIGURE: 1 / 6
FOXO4 Antibody (PA5-20974) in ICC/IF
Immunofluorescence analysis of FOXO4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FOXO4 Polyclonal Antibody (Product # PA5-20974) at 10 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More
Product Details
PA5-20974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 16 amino acid peptide near the amino terminus of human FOXO4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is Hela cell lysate.
PA5-20974 can be used with blocking peptide PEP-1088.
Target Information
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. It is also known as AFX1 or MLLT7 (Myeloid/Lymphoid or Mixed-Lineage Leukemia (Trithorax Homolog, Translocated To, 7) and is a member of the O class of forkhead/ winged helix family of transcription factors along with FOXO1, FOXO3 and FOXO6. FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. FOXO4 binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1. However, the activation of another downstream target of the Insulin/IGF1 pathway, Akt, leads to phosphorylation of FOXO4 on specific Ser/Thr residues. This phosphorylation mediates binding of FOXO4 to 14-3-3 and eventual exit of FOXO4 from the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AFX; AFX1; Afxh; AFXzeta; Fork head domain transcription factor AFX1; Forkhead box protein O4; forkhead protein; FOX04b; FOXO4b; MGC120490; myeloid/lymphoid or mixed lineage-leukemia translocation to 7 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; OTTHUMP00000023497; unnamed protein product
Gene Aliases: AFX; AFX1; Afxh; Fkhr3; FOXO4; MLLT7; RGD1561201
UniProt ID: (Human) P98177, (Mouse) Q9WVH3
Entrez Gene ID: (Human) 4303, (Rat) 302415, (Mouse) 54601
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
beta-catenin binding
enzyme binding
identical protein binding
sequence-specific DNA binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
transcription factor binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cell cycle arrest
mitotic G2 DNA damage checkpoint
negative regulation of cell proliferation
insulin receptor signaling pathway
negative regulation of angiogenesis
positive regulation of transcription, DNA-templated
stem cell differentiation
negative regulation of smooth muscle cell differentiation
negative regulation of G0 to G1 transition
response to oxidative stress
muscle organ development
positive regulation of smooth muscle cell migration
cell differentiation
response to nutrient levels
cellular response to oxidative stress
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
protein K48-linked ubiquitination
response to water-immersion restraint stress
transcription, DNA-templated
cell cycle
multicellular organism development
anatomical structure morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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