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          • Primary Antibodies ›
          • GCP2 Antibodies

          Invitrogen

          GCP2 Polyclonal Antibody

          View all (5) GCP2 antibodies

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          Cite GCP2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          GCP2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GCP2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          GCP2 Antibody (PA5-21433) in ICC/IF

          GCP2 Polyclonal Antibody detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBGCP2 protein stained by GCP2 Polyclonal Antibody (Product # PA5-21433) diluted at 1:500. Blue: Hoechst 33342 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          GCP2 Antibody in Immunocytochemistry (ICC/IF)
          GCP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GCP2 Antibody in Western Blot (WB)
          GCP2 Antibody in Western Blot (WB)
          GCP2 Polyclonal Antibody

          Product Details

          PA5-21433

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          - -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the N-terminus region of human GCP2. The exact sequence is proprietary.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 10% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11154079

          Product Specific Information

          Recommended positive controls: A431, HeLa, NIH-3T3.

          Predicted reactivity: Mouse (88%), Rat (88%), Bovine (87%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The γ-Tubulin complex is composed of γ Tubulin and the γ-Tubulin complexassociated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. γ-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the γ-Tubulin complex. GCP2 (γ-Tubulin complex component 2), also known as TUBGCP2 or SPBC97 (spindle pole body protein Spc97 homolog) is a ubiquitously expressed 902 amino acid protein that localizes to the centrosome and is involved in microtubule nucleation.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: component of cytoplasmic gamma-tubulin complex; Gamma-ring complex protein 103 kDa; Gamma-tubulin complex component 2; GCP-2; h103p; hGrip103; hSpc97; Spindle pole body protein Spc97 homolog; tubulin gamma complex associated protein 2; unnamed protein product

          View more View less

          Gene Aliases: 1700022B05Rik; ALP4; GCP-2; GCP2; Grip103; h103p; hGCP2; hSpc97; PAMDDFS; SPBC97; SPC97; Spc97p; TUBGCP2

          View more View less

          UniProt ID: (Human) Q9BSJ2, (Mouse) Q921G8

          View more View less

          Entrez Gene ID: (Human) 10844, (Mouse) 74237

          View more View less

          Function(s)
          protein binding gamma-tubulin binding microtubule minus-end binding molecular_function non-motor microtubule binding protein
          Process(es)
          microtubule cytoskeleton organization mitotic cell cycle neuron migration microtubule nucleation brain development cytoplasmic microtubule organization spindle assembly meiotic cell cycle protein-containing complex assembly biological_process
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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