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Invitrogen
AMPK alpha-2 Polyclonal Antibody
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Product Details
PA5-21494
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human AMPK alpha 2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.96 mg/mL
Amount
96 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3.
Predicted reactivity: Mouse (98%), Rat (98%), Pig (99%), Chicken (96%), Sheep (98%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 variant B; AMPK alpha 2; AMPK alpha-2 chain; AMPK alpha-2 variant 2; AMPK alpha-2 variant 3; AMPK subunit alpha-2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; prkaa2; protein kinase; protein kinase, AMP-activated, alpha 2 catalytic subunit; unnamed protein product
Gene Aliases: 2310008I11Rik; A830082D05; AMPK; AMPK2; AMPKa2; AMPKalpha2; PRKAA; PRKAA2
UniProt ID: (Human) P54646, (Rat) Q09137, (Mouse) Q8BRK8
Entrez Gene ID: (Human) 5563, (Rat) 78975, (Mouse) 108079
nucleotide binding
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
AMP-activated protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
metal ion binding
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
protein serine kinase activity
histone H2BS36 kinase activity
protein binding, bridging
histone serine kinase activity
[acetyl-CoA carboxylase] kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
cytoplasmic translation
chromatin organization
chromatin remodeling
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
phosphatidylethanolamine biosynthetic process
phosphatidylcholine biosynthetic process
steroid biosynthetic process
cholesterol biosynthetic process
autophagy
signal transduction
steroid metabolic process
cholesterol metabolic process
lipid biosynthetic process
cellular response to starvation
regulation of gene expression
positive regulation of autophagy
negative regulation of gene expression
response to muscle activity
Wnt signaling pathway
sterol biosynthetic process
positive regulation of macroautophagy
regulation of macroautophagy
cellular response to nutrient levels
negative regulation of TOR signaling
cellular response to amino acid starvation
cellular response to oxidative stress
TORC1 signaling
cellular response to glucose starvation
glucose homeostasis
regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of glycolytic process
negative regulation of translational initiation
positive regulation of translational initiation
rhythmic process
fatty acid homeostasis
protein localization to lysosome
regulation of stress granule assembly
regulation of microtubule cytoskeleton organization
cellular response to calcium ion
cellular response to glucose stimulus
cellular response to prostaglandin E stimulus
cellular response to xenobiotic stimulus
protein K6-linked ubiquitination
positive regulation of release of cytochrome c from mitochondria
energy homeostasis
hepatocyte apoptotic process
positive regulation of cellular protein localization
negative regulation of hepatocyte apoptotic process
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
negative regulation of tubulin deacetylation
lipid droplet disassembly
protein localization to lipid particle
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
response to activity
regulation of lipid metabolic process
response to caffeine
histone-serine phosphorylation
protein heterooligomerization
cellular response to organonitrogen compound
regulation of energy homeostasis
response to stress
phosphorylation
chromatin modification
intracellular signal transduction
cellular response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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