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Invitrogen
NuMA Polyclonal Antibody
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FIGURE: 1 / 11
NuMA Antibody (PA5-22285) in ICC/IF
Immunofluorescence analysis of NuMA was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NuMA Polyclonal Antibody (Product # PA5-22285) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1... View More
Product Details
PA5-22285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human NuMA. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Amount
54 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, HeLa, HeLa (100 ng/mL Nocodazole treatment for 24 hr).
Predicted reactivity: Mouse (81%), Rat (83%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein; unnamed protein product
Gene Aliases: NMP-22; NMP22; NUMA; NUMA1
UniProt ID: (Human) Q14980
Entrez Gene ID: (Human) 4926
structural molecule activity
protein binding
microtubule binding
lipid binding
tubulin binding
protein domain specific binding
phosphatidylinositol binding
macromolecular complex binding
microtubule plus-end binding
microtubule minus-end binding
dynein complex binding
disordered domain specific binding
establishment of mitotic spindle orientation
microtubule bundle formation
nucleus organization
chromosome segregation
positive regulation of BMP signaling pathway
astral microtubule organization
positive regulation of microtubule polymerization
positive regulation of intracellular transport
positive regulation of keratinocyte differentiation
cell division
meiotic cell cycle
positive regulation of hair follicle development
positive regulation of chromosome segregation
anastral spindle assembly
regulation of mitotic spindle organization
regulation of metaphase plate congression
positive regulation of protein localization to spindle pole body
positive regulation of mitotic spindle elongation
positive regulation of protein localization to cell cortex
positive regulation of chromosome separation
positive regulation of spindle assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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