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Invitrogen
HNF1A Polyclonal Antibody
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FIGURE: 1 / 5
HNF1A Antibody (PA5-22310) in ICC/IF
HNF1A Polyclonal Antibody detects HNF1 alpha protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha stained by HNF1A Polyclonal Antibody (Product # PA5-22310) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Product Details
PA5-22310
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.27 mg/mL
Amount
127 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HepG2, Mouse liver, Rat liver.
Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (83%), Pig (99%), Chicken (95%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: albumin proximal factor; hepatic nuclear factor 1; hepatocyte nuclear factor 1; Hepatocyte nuclear factor 1-alpha; hnf-1 alpha; HNF-1-alpha; HNF-1A; interferon production regulator factor; LFB1; Liver-specific transcription factor LF-B1; TCF-1; transcription factor; Transcription factor 1; transcription factor 1, hepatic; unnamed protein product
Gene Aliases: AI323641; Hnf-1; HNF-1-alpha; HNF-1A; HNF1; HNF1-alpha; HNF1[a]; HNF1A; HNF1alpha; HNF4A; IDDM20; LFB1; MODY3; TCF-1; TCF1
UniProt ID: (Human) P20823, (Mouse) P22361
Entrez Gene ID: (Human) 6927, (Mouse) 21405
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein homodimerization activity
protein heterodimerization activity
protein dimerization activity
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription cofactor binding
double-stranded DNA binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
DNA-binding transcription factor
liver development
regulation of transcription from RNA polymerase II promoter
insulin secretion
pancreas development
regulation of pronephros size
renal glucose absorption
glucose homeostasis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
glucose import
positive regulation of transcription initiation from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter
blastocyst development
placenta development
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
fatty acid biosynthetic process
bile acid biosynthetic process
heme biosynthetic process
response to oxidative stress
protein localization
cholesterol metabolic process
response to glucose
bile acid and bile salt transport
fatty acid transport
regulation of Wnt signaling pathway
embryonic limb morphogenesis
endocrine pancreas development
reverse cholesterol transport
bone resorption
regulation of hormone secretion
paraxial mesoderm formation
reproductive structure development
regulation of insulin secretion
SMAD protein signal transduction
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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