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Invitrogen
PNPase Polyclonal Antibody
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Product Details
PA5-22396
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PNPase. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji, mouse liver.
Predicted reactivity: Human (99%), Mouse (93%), Rat (95%), Pig (94%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PNPT1 (polyribonucleotide nucleotidyltransferase 1, mitochondirla) is a RNA-binding protein implicated in numerous RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. It is required for correct processing and polyadenylation of mitochondrial mRNAs. It plays a role as a cytoplasmic RNA import factor, in mitochondrial morphogenesis and respiration, regulation of expression of the electron transport chain, regulation of the stability of specific mature miRNAs in melanoma cells, and RNA cell surveilance. Mutations of the gene can result in combined oxidative phosphorylation deficiency 13 (COXPD13) and deafness, autosomal recessive, 70 (DFNB70).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3'-5' RNA exonuclease OLD35; DKFZp762K1914; old 35; Old-35; PNPase 1; PNPase old-35; Polynucleotide phosphorylase 1; Polynucleotide phosphorylase-like protein; Polyribonucleotide nucleotidyltransferase 1, mitochondrial
Gene Aliases: 1200003F12Rik; COXPD13; DFNB70; old-35; OLD35; PNPASE; PNPT1; Pnptl1; SCA25
UniProt ID: (Human) Q8TCS8, (Mouse) Q8K1R3
Entrez Gene ID: (Human) 87178, (Mouse) 71701
3'-5'-exoribonuclease activity
nucleic acid binding
RNA binding
nuclease activity
exonuclease activity
polyribonucleotide nucleotidyltransferase activity
protein binding
poly(U) RNA binding
transferase activity
nucleotidyltransferase activity
hydrolase activity
poly(G) binding
miRNA binding
identical protein binding
poly(A) RNA binding
nucleotidyltransferase
mitochondrial RNA catabolic process
mitochondrial mRNA catabolic process
positive regulation of mitochondrial RNA catabolic process
mitochondrial RNA 5'-end processing
mitochondrial RNA 3'-end processing
RNA processing
mRNA processing
RNA catabolic process
mRNA catabolic process
mitochondrion organization
cellular response to oxidative stress
cellular response to interferon-beta
RNA import into mitochondrion
rRNA import into mitochondrion
regulation of cellular respiration
protein homooligomerization
response to cAMP
response to growth hormone
positive regulation of mRNA catabolic process
protein homotrimerization
nuclear polyadenylation-dependent mRNA catabolic process
mitochondrial mRNA polyadenylation
liver regeneration
positive regulation of miRNA catabolic process
regulation of cellular senescence
transport
RNA polyadenylation
negative regulation of growth
mitochondrion morphogenesis
mitotic cell cycle arrest
RNA phosphodiester bond hydrolysis, exonucleolytic
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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