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Invitrogen
MYOD Polyclonal Antibody
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FIGURE: 1 / 18
MYOD Antibody (PA5-23078) in ICC/IF
Immunocytochemistry analysis of MYOD in RD cells. Samples were incubated with MYOD polyclonal antibody (Product # PA5-23078) using a dilution of 5 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Product Details
PA5-23078
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150).
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The target sequence has 90% sequence homology with mouse.
Target Information
The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; MyoD; Myogenic factor 3; unnamed protein product
Gene Aliases: AI503393; BHLHC1; CMYO17; CMYP17; MYF3; MYOD; Myod-1; MYOD1; MYODRIF; PUM
UniProt ID: (Human) P15172, (Mouse) P10085
Entrez Gene ID: (Human) 4654, (Mouse) 17927
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
ligand-dependent nuclear receptor binding
enzyme binding
chromatin DNA binding
ubiquitin protein ligase binding
protein homodimerization activity
bHLH transcription factor binding
sequence-specific DNA binding
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
core promoter binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor binding
nuclear hormone receptor binding
protein heterodimerization activity
basic helix-loop-helix transcription factor
regulation of alternative mRNA splicing, via spliceosome
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
muscle organ development
myoblast fate determination
skeletal muscle tissue development
myoblast fusion
cellular response to starvation
tissue development
regulation of gene expression
myotube differentiation
myotube cell development
myotube differentiation involved in skeletal muscle regeneration
cell differentiation
skeletal muscle cell differentiation
muscle cell differentiation
muscle cell fate commitment
skeletal muscle tissue regeneration
positive regulation of skeletal muscle tissue regeneration
regulation of RNA splicing
skeletal muscle fiber adaptation
myoblast differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
animal organ development
skeletal muscle fiber development
positive regulation of skeletal muscle fiber development
striated muscle cell differentiation
positive regulation of muscle cell differentiation
cellular response to tumor necrosis factor
cellular response to glucocorticoid stimulus
cellular response to estradiol stimulus
cellular response to oxygen levels
positive regulation of myoblast fusion
positive regulation of snRNA transcription by RNA polymerase II
negative regulation of myoblast proliferation
transcription, DNA-templated
multicellular organism development
histone H3 acetylation
histone H4 acetylation
cardiac muscle cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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